Wnt signaling in periodontitis

被引：6

作者：

Guney, Zeliha ^{[1
,2
]}

Kurgan, Sivge ^{[1
]}

Onder, Canan ^{[1
]}

Tayman, Mahmure Ayse ^{[3
]}

Gunhan, Oemer ^{[4
]}

Kantarci, Alpdogan ^{[5
]}

Serdar, Muhittin Abdulkadir ^{[6
]}

Gunhan, Meral ^{[1
]}

机构：

[1] Ankara Yildirim Beyazit Univ, Fac Dent, Dept Periodontol, Ankara, Turkiye

[2] Ankara Medipol Univ, Fac Dent, Dept Periodontol, Ankara, Turkiye

[3] Yildirim Beyazit Univ, Fac Dent, Dept Periodontol, Ankara, Turkiye

[4] TOBB Univ, Fac Med, Dept Pathol, Ankara, Turkiye

[5] Forsyth Inst, Cambridge, MA USA

[6] Acibadem Univ, Fac Med, Dept Biochem, Istanbul, Turkiye

来源：

CLINICAL ORAL INVESTIGATIONS | 2023年 / 27卷 / 11期

关键词：

Periodontitis; Wnt; 3a; 5a; 10b; beta-Catenin; GENE-EXPRESSION; WNT/BETA-CATENIN; WINGLESS HOMOLOG; BETA-CATENIN; BONE; PROTEIN; CELLS; TRANSCRIPTION; PROMOTES; DIFFERENTIATION;

D O I：

10.1007/s00784-023-05294-7

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

ObjectiveThis study aimed to evaluate the Wnt/beta-catenin signaling pathway activity in gingival samples obtained from patients with periodontitis.Materials and methodsFifteen patients with stage III grade B (SIIIGB) and eleven with stage III grade C (SIIIGC) periodontitis were included and compared to 15 control subjects. beta-Catenin, Wnt 3a, Wnt 5a, and Wnt 10b expressions were evaluated by Q-PCR. Topographic localization of tissue beta-catenin, Wnt 5a, and Wnt 10b was measured by immunohistochemical analysis. TNF-alpha was used to assess the inflammatory state of the tissues, while Runx2 was used as a mediator of active destruction.ResultsWnt 3a, Wnt 5a, and Wnt 10b were significantly higher in gingival tissues in both grades of stage 3 periodontitis compared to the control group (p < 0.05). beta-Catenin showed intranuclear staining in connective tissue in periodontitis, while it was confined to intracytoplasmic staining in epithelial tissue and the cell walls in the control group. Wnt5a protein expression was elevated in periodontitis, with the most intense staining observed in the connective tissue of SIIIGC samples. Wnt10b showed the highest density in the connective tissue of patients with periodontitis.ConclusionsOur findings suggested that periodontal inflammation disrupts the Wnt/beta-catenin signaling pathway.Clinical RelevancePeriodontitis disrupts Wnt signaling in periodontal tissues in parallel with tissue inflammation and changes in morphology. This change in Wnt-related signaling pathways that regulate tissue homeostasis in the immunoinflammatory response may shed light on host-induced tissue destruction in the pathogenesis of the periodontal disease.

引用

页码：6801 / 6812

页数：12

共 64 条

[1] Gingival crevicular fluid galectin-3 and interleukin-1 beta levels in stage 3 periodontitis with grade B and C [J].

Afacan, Beral ;

Ilhan, Harika Atmaca ;

Kose, Timur ;

Emingil, Gulnur .

CLINICAL ORAL INVESTIGATIONS, 2023, 27 (07) :3749-3758

[2] Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling [J].

Alizadeh, AA ;

Eisen, MB ;

Davis, RE ;

Ma, C ;

Lossos, IS ;

Rosenwald, A ;

Boldrick, JG ;

Sabet, H ;

Tran, T ;

Yu, X ;

Powell, JI ;

Yang, LM ;

Marti, GE ;

Moore, T ;

Hudson, J ;

Lu, LS ;

Lewis, DB ;

Tibshirani, R ;

Sherlock, G ;

Chan, WC ;

Greiner, TC ;

Weisenburger, DD ;

Armitage, JO ;

Warnke, R ;

Levy, R ;

Wilson, W ;

Grever, MR ;

Byrd, JC ;

Botstein, D ;

Brown, PO ;

Staudt, LM .

NATURE, 2000, 403 (6769) :503-511

[3]

Arabaci Taner, 2010, Eur J Dent, V4, P454

[4]

Armitage G C, 1999, Ann Periodontol, V4, P1, DOI 10.1902/annals.1999.4.1.1

[5] Immunohistochemical analysis of inflammatory infiltrate in aggressive and chronic periodontitis: a comparative study [J].

Artese, Luciano ;

Simon, Maciej J. ;

Piattelli, Adriano ;

Ferrari, Daniel S. ;

Cardoso, Luciana A. G. ;

Faveri, Marcelo ;

Onuma, Tatiana ;

Piccirilli, Marcello ;

Perrotti, Vittoria ;

Shibli, Jamil A. .

CLINICAL ORAL INVESTIGATIONS, 2011, 15 (02) :233-240

[6] Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation [J].

Baksh, Dolores ;

Boland, Genevieve M. ;

Tuan, Rocky S. .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 101 (05) :1109-1124

[7] Regulation of osteoblastogenesis and bone mass by Wnt10b [J].

Bennett, CN ;

Longo, KA ;

Wright, WS ;

Suva, LJ ;

Lane, TF ;

Hankenson, KD ;

MacDougald, OA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (09) :3324-3329

[8] Granulation Tissue Eroding the Subchondral Bone Also Promotes New Bone Formation in Ankylosing Spondylitis [J].

Bleil, Janine ;

Maier, Rene ;

Hempfing, Axel ;

Sieper, Joachim ;

Appel, Heiner ;

Syrbe, Uta .

ARTHRITIS & RHEUMATOLOGY, 2016, 68 (10) :2456-2465

[9] The Wingless homolog, WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation [J].

Blumenthal, Antje ;

Ehlers, Stefan ;

Lauber, Jorg ;

Buer, Jan ;

Lange, Christoph ;

Goldmann, Torsten ;

Heine, Holger ;

Brandt, Ernst ;

Reiling, Norbert .

BLOOD, 2006, 108 (03) :965-973

[10] Osteocytes, mechanosensing and Wnt signaling [J].

Bonewald, Lynda F. ;

Johnson, Mark L. .

BONE, 2008, 42 (04) :606-615

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