Structural Analysis of the Colony-Stimulating Factor 3 Gene of Granulocyte Colony-Stimulating Factor-Producing Urothelial Cancer

被引:0
作者
Okuno, Yumiko [1 ]
Hori, Mai [1 ]
-Kato, Mami Hattori [1 ]
Fukuhara, Hiroshi [2 ]
Nomiya, Akira [1 ]
Mikami, Koji [1 ]
Takeuchi, Takumi [1 ]
机构
[1] Kanto Rosai Hosp, Dept Urol, Japan Org Occupat Hlth & Safety, Kawasaki, Japan
[2] Kyorin Univ, Fac Med, Dept Urol, Tokyo, Japan
关键词
transcription; mutation; csf3; g-csf; urothelial cancer; CARCINOMA; PEMBROLIZUMAB; EXPRESSION; CDNA;
D O I
10.7759/cureus.43981
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate the proliferation, differentiation, and mobilization of neutrophils. G-CSF-producing malignant cancers have been reported to occur in various organs and are mostly associated with poor clinical prognosis. Here, we analyzed the structure of the CSF3 gene encoding the G-CSF protein to delineate the mechanism of G-CSF production by the cancer cells. Methodology Two cases of G-CSF-producing urothelial cancers and three cases of G-CSF-nonproducing bladder cancers were enrolled for genetic analysis. Results In one case of G-CSF-producing bladder cancer, six somatic mutations were detected in the 5'-upstream region of the CSF3 gene. No somatic mutations in the CSF3 gene were detected in another case of G-CSF-producing renal pelvic cancer and G-CSF-nonproducing bladder cancers. Copy numbers of the CSF3 gene were not increased in G-CSF-producing urothelial cancers. Conclusions Somatic mutations in the 5'-upstream region of the CSF3 gene may cause G-CSF protein overproduction.
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页数:9
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