Gastric Cancer Derived Mesenchymal Stem Cells Promote the Migration of Gastric Cancer Cells Through miR-374a-5p

被引：11
作者：
Ji, Runbi [1 ,2 ]
Lin, Jiang [3 ]
Gu, Hongbing [1 ]
Ma, Jichun [3 ]
Fu, Min [3 ]
Zhang, Xu [2 ]
机构：
[1] Jiangsu Univ, Dept Clin Lab, Affiliated Peoples Hosp, Zhenjiang 212002, Jiangsu, Peoples R China
[2] Jiangsu Univ, Sch Med, Jiangsu Key Lab Med Sci & Lab Med, Zhenjiang 212013, Jiangsu, Peoples R China
[3] Jiangsu Univ, Affiliated Peoples Hosp, Dept Cent Lab, Zhenjiang 212002, Peoples R China
来源：
CURRENT STEM CELL RESEARCH & THERAPY | 2023年 / 18卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Mesenchymal stem cell; gastric cancer; migration; miR-374a-5p; tumor microenvironment; HAPLN1; INVASION; PROLIFERATION; PROGRESSION;
D O I：
10.2174/1574888X18666221124145847
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Introduction: Resident mesenchymal stem cells (MSCs) in the tumor microenvironment play an important role in tumor progression. Up to now, the mechanism of resident MSCs promoting gastric cancer cell migration remains unclear. Methods: We tested the migration ability of gastric cancer cells by transwell assays in this study. The inflammatory factors secreted by MSCs were detected by Luminex and ELISA. The activation of NF-& kappa;B signaling was detected by western blot. The exosomes derived from MSCs were isolated and identified by transmission electron microscope, nano-sight and western blot. The expression of miR-374a-5p was confirmed by qRT-PCR and its downstream target HAPLN1 by luciferase reporter assay. The expression of adhesion molecules of gastric cancer cells was detected by flow cytometry. Results: MiR-374a-5p could regulate the expression of inflammatory factors by activating NF-& kappa;B signaling. The increase of MCP-1 and the decrease of IFN-& gamma; promoted the migration of gastric cancer cells. The miR-374a-5p in MSCs could be encapsulated and delivered to gastric cancer cells by exosomes derived from MSCs. Exogenous miR-374a-5p up-regulated the expression of adhesion molecules in gastric cancer cells by targeting HAPLN1. And miR-374a-5p-enriched exosomes also promoted the migration of gastric cancer cells. Conclusion: MiR-374a-5p promoted gastric cancer metastasis, and resident MSCs in the gastric cancer microenvironment played a major role in the regulation of gastric cancer metastasis. The study will provide new ideas and potential targets for the prevention and treatment of gastric cancer metastasis.
引用
收藏
页码：853 / 863
页数：11
相关论文
共 35 条
[1]   Acetyl-L-Carnitine downregulates invasion (CXCR4/CXCL12, MMP-9) and angiogenesis (VEGF, CXCL8) pathways in prostate cancer cells: rationale for prevention and interception strategies [J].
Baci, Denisa ;
Bruno, Antonino ;
Cascini, Caterina ;
Gallazzi, Matteo ;
Mortara, Lorenzo ;
Sessa, Fausto ;
Pelosi, Giuseppe ;
Albini, Adriana ;
Noonan, Douglas M. .
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2019, 38 (01)
[2]   The latest progress on miR-374 and its functional implications in physiological and pathological processes [J].
Bian, Hongjun ;
Zhou, Yi ;
Zhou, Dawei ;
Zhang, Yongsheng ;
Shang, Deya ;
Qi, Jianni .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (05) :3063-3076
[3]   RETRACTED: Exosomes Derived from miR-143-Overexpressing MSCs Inhibit Cell Migration and Invasion in Human Prostate Cancer by Downregulating TFF3 (Retracted article. See vol. 31, pg. 28, 2023) [J].
Che, Yuanyuan ;
Shi, Xu ;
Shi, Yunpeng ;
Jiang, Xiaoming ;
Ai, Qing ;
Shi, Ying ;
Gong, Fengyan ;
Jiang, Wenyan .
MOLECULAR THERAPY-NUCLEIC ACIDS, 2019, 18 :232-244
[4]   Modifying the tumour microenvironment and reverting tumour cells: New strategies for treating malignant tumours [J].
Cheng, Ya Qi ;
Wang, Shou Bi ;
Liu, Jia Hui ;
Jin, Lin ;
Liu, Ying ;
Li, Chao Yang ;
Su, Ya Ru ;
Liu, Yu Run ;
Sang, Xuan ;
Wan, Qi ;
Liu, Chang ;
Yang, Liu ;
Wang, Zhi Chong .
CELL PROLIFERATION, 2020, 53 (08)
[5]   Loss of androgen receptor signaling in prostate cancer-associated fibroblasts (CAFs) promotes CCL2-and CXCL8-mediated cancer cell migration [J].
Cioni, Bianca ;
Nevedomskaya, Ekaterina ;
Melis, Monique H. M. ;
van Burgsteden, Johan ;
Stelloo, Suzan ;
Hodel, Emma ;
Spinozzi, Daniele ;
de Jong, Jeroen ;
van der Poel, Henk ;
de Boer, Jan Paul ;
Wessels, Lodewyk F. A. ;
Zwart, Wilbert ;
Bergman, Andries M. .
MOLECULAR ONCOLOGY, 2018, 12 (08) :1308-1323
[6]   Advanced gastric cancer: Current treatment landscape and future perspectives [J].
Digklia, Antonia ;
Wagner, Anna Dorothea .
WORLD JOURNAL OF GASTROENTEROLOGY, 2016, 22 (08) :2403-2414
[7]   Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis [J].
Ecker, Brett L. ;
Kaur, Amanpreet ;
Douglass, Stephen M. ;
Webster, Marie R. ;
Almeida, Filipe, V ;
Marino, Gloria E. ;
Sinnamon, Andrew J. ;
Neuwirth, Madalyn G. ;
Alicea, Gretchen M. ;
Ndoye, Abibatou ;
Fane, Mitchell ;
Xu, Xiaowei ;
Sim, Myung Shin ;
Deutsch, Gary B. ;
Faries, Mark B. ;
Karakousis, Giorgos C. ;
Weeraratna, Ashani T. .
CANCER DISCOVERY, 2019, 9 (01) :82-95
[8]   Hepatoma cell-secreted exosomal microRNA-103 increases vascular permeability and promotes metastasis by targeting junction proteins [J].
Fang, Jian-Hong ;
Zhang, Zi-Jun ;
Shang, Li-Ru ;
Luo, Yu-Wei ;
Lin, Yi-Fang ;
Yuan, Yunfei ;
Zhuang, Shi-Mei .
HEPATOLOGY, 2018, 68 (04) :1459-1475
[9]   Interferon γ suppresses dentin sialophosphoprotein in oral squamous cell carcinoma cells resulting in antitumor effects, via modulation of the endoplasmic reticulum response [J].
Gkouveris, Ioannis ;
Nikitakis, Nikolaos G. ;
Aseervatham, Jaya ;
Ogbureke, Kalu U. E. .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2018, 53 (06) :2423-2432
[10]   Role of MSC in the Tumor Microenvironment [J].
Hass, Ralf .
CANCERS, 2020, 12 (08) :1-17
1234