Cyanidin-3-O-glucoside protects intestinal epithelial cells from palmitate-induced lipotoxicity

被引:27
作者
Bashllari, Romina [1 ]
Molonia, Maria Sofia [1 ,2 ]
Muscara, Claudia [1 ]
Speciale, Antonio [1 ]
Wilde, Peter J. [3 ]
Saija, Antonella [1 ]
Cimino, Francesco [1 ]
机构
[1] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Messina, Italy
[2] Univ Messina, Prof Antonio Imbesi Fdn, Messina, Italy
[3] Quadram Inst Biosci, Food Innovat & Hlth Programme, Norwich Res Pk, Norwich, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
Anthocyanins; IBD; intestinal inflammation; lipotoxicity; Nrf2; NF-KAPPA-B; INFLAMMATORY-BOWEL-DISEASE; INDUCED ENDOTHELIAL DYSFUNCTION; TNF-ALPHA; NRF2; ANTHOCYANINS; ACID; CYANIDIN-3-GLUCOSIDE; METABOLISM; MODULATION;
D O I
10.1080/13813455.2020.1828480
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Context Increased free fatty acids (FFAs) levels, typical in obesity condition, can contribute to systemic lipotoxicity and inflammation adversely influencing Inflammatory Bowel Disease development and progression. Anthocyanins possess health promoting properties mainly associated to the induction of Nrf2-regulated cytoprotective proteins. Objective Using a novel experimental model, we evaluated the in vitro intracellular mechanisms involved in FFAs modulation of intestinal epithelial lipotoxicity and the protective effects of cyanidin-3-O-glucoside (C3G) in Caco-2 cells. Results Caco-2 exposed to palmitic acid (PA) in the serosal (basolateral) side showed a combined state of epithelial inflammation, inducing NF-kappa B pathway and downstream cytokines, that was reverted by C3G apical pre-treatment. In addition, PA altered intracellular redox status and induced reactive oxygen species that were reduced by C3G via the redox-sensitive Nrf2 signalling. Discussion and conclusion Results suggest that anti-inflammatory properties of anthocyanins, mediated by Nrf2, could represent an interesting tool for intestinal inflammatory disorders.
引用
收藏
页码:379 / 386
页数:8
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