Silk fibroin, gelatin, and human placenta extracellular matrix-based composite hydrogels for 3D bioprinting and soft tissue engineering

被引：18

作者：

Schneider, Karl Heinrich ^{[1
,2
,3
]}

Goldberg, Benjamin J. ^{[3
]}

Hasturk, Onur ^{[3
]}

Mu, Xuan ^{[3
,4
]}

Doetzlhofer, Marvin ^{[1
]}

Eder, Gabriela ^{[1
]}

Theodossiou, Sophia ^{[3
,5
]}

Pichelkastner, Luis ^{[1
]}

Riess, Peter ^{[1
]}

Rohringer, Sabrina ^{[1
]}

Kiss, Herbert ^{[6
]}

Teuschl-Woller, Andreas H. ^{[7
]}

Fitzpatrick, Vincent ^{[3
,8
]}

Enayati, Marjan ^{[1
,2
]}

Podesser, Bruno K. ^{[1
,2
]}

Bergmeister, Helga ^{[1
,2
]}

Kaplan, David L. ^{[3
]}

机构：

[1] Med Univ Vienna, Ctr Biomed Res & Translat Surg, A-1090 Vienna, Austria

[2] Ludwig Boltzmann Inst Cardiovasc Res, A-1090 Vienna, Austria

[3] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA

[4] Univ Iowa, Coll Engn, Roy J Carver Dept Biomed Engn, Iowa City, IA 52242 USA

[5] Boise State Univ, Dept Mech & Biomed Engn, Boise, ID 83725 USA

[6] Med Univ Vienna, Dept Obstet & Gynecol, Div Obstet & Fetomaternal Med, A-1090 Vienna, Austria

[7] Univ Appl Sci Tech Wien, Dept Life Sci Technol, A-1200 Vienna, Austria

[8] Univ Technol Compiegne, Sorbonne Univ, UMR CNRS Biomech & Bioengn 7338, F-60203 Compiegne, France

来源：

BIOMATERIALS RESEARCH | 2023年 / 27卷 / 01期

关键词：

SCAFFOLD DESIGN; IMPACT;

D O I：

10.1186/s40824-023-00431-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

BackgroundThere is a great clinical need and it remains a challenge to develop artificial soft tissue constructs that can mimic the biomechanical properties and bioactivity of natural tissue. This is partly due to the lack of suitable biomaterials. Hydrogels made from human placenta offer high bioactivity and represent a potential solution to create animal-free 3D bioprinting systems that are both sustainable and acceptable, as placenta is widely considered medical waste. A combination with silk and gelatin polymers can bridge the biomechanical limitations of human placenta chorion extracellular matrix hydrogels (hpcECM) while maintaining their excellent bioactivity.MethodIn this study, silk fibroin (SF) and tyramine-substituted gelatin (G-TA) were enzymatically crosslinked with human placental extracellular matrix (hpcECM) to produce silk-gelatin-ECM composite hydrogels (SGE) with tunable mechanical properties, preserved elasticity, and bioactive functions. The SGE composite hydrogels were characterized in terms of gelation kinetics, protein folding, and bioactivity. The cyto- and biocompatibility of the SGE composite was determined by in vitro cell culture and subcutaneous implantation in a rat model, respectively. The most cell-supportive SGE formulation was then used for 3-dimensional (3D) bioprinting that induced chemical crosslinking during extrusion.ConclusionAddition of G-TA improved the mechanical properties of the SGE composite hydrogels and inhibited crystallization and subsequent stiffening of SF for up to one month. SGE hydrogels exhibit improved and tunable biomechanical properties and high bioactivity for encapsulated cells. In addition, its use as a bioink for 3D bioprinting with free reversible embedding of suspended hydrogels (FRESH) has been validated, opening the possibility to fabricate highly complex scaffolds for artificial soft tissue constructs with natural biomechanics in future.

引用

页数：19