Postharvest application of carrageenan oligosaccharides on quality attributes and activities of defense-related enzymes in strawberry (Fragaria x ananassa Duch)

被引:5
作者
Guo, Zilong [1 ,2 ]
Ning, Limin [1 ]
Yao, Zhong [2 ]
Zhu, Benwei [2 ]
机构
[1] Nanjing Univ Chinese Med, Sch Med & Holist Integrated Med, Nanjing 210023, Peoples R China
[2] Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Peoples R China
基金
中国国家自然科学基金;
关键词
Carrageenan oligosaccharides; Strawberry preservation; Storage; Mechanism; SHELF-LIFE; ACID TREATMENT; GENE-EXPRESSION; ASCORBIC-ACID; FUNGAL DECAY; CHITOSAN; FRUIT; IMPROVEMENT; PREHARVEST; RESISTANCE;
D O I
10.1016/j.bcab.2023.102893
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The carrageenan oligosaccharides (COS) possessed versatile biological activities such as antibacterial, antioxidant, anti-inflammatory activities. Herein, we aimed to explore the effects of carrageenan oligosaccharides (COS) on the preservation of strawberries (Fragariaxananassa Duch) and factors including decay percentage, hardness, soluble solids, titratable acids, vitamin C (VC) content, the activity of peroxidase (POD), polyphenol oxidase (PPO) were judged as criteria. Also, the expression of genes related to fruit-softening enzymes were detected by real-time qPCR. Soaking treatment of oligosaccharides could significantly (P < 0.05) improve the physical and chemical factors compared with the control group. As for the preservation mechanism of strawberries, the Real-time qPCR results showed that COS coating treatment significantly prolonged the shelf life of strawberries by suppressing the gene expression of cell wall degraded enzymes and ethylene biosynthesis enzymes, with the most obvious effects observed in the 10 mg/mL concentration group. This experiment explores the possibility of COS as a strawberry preservative, which lays a theoretical foundation for future practical application.
引用
收藏
页数:11
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