Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil

被引：0

作者：

Manzini, Suzane ^{[1
]}

Bertozzo, Thaina Valente ^{[1
]}

Aires, Isabella Neves ^{[1
]}

Rodrigues, Nassarah Jabur Lot ^{[1
]}

Bertolini, Amanda Bezerra ^{[2
]}

Alexandrino, Marcela ^{[1
]}

Steinle, Jackieline Sampaio ^{[2
]}

de Melo, Renata Pimentel Bandeira ^{[3
]}

Mota, Rinaldo Aparecido ^{[3
]}

de Medeiros, Maria Izabel Merino ^{[4
]}

Richini-Pereira, Virginia Bodelao ^{[5
]}

Curci, Vera Claudia Lorenzetti Magalhaes ^{[6
]}

Lucheis, Simone Baldini ^{[1
,2
,4
]}

机构：

[1] Sao Paulo State Univ UNESP, Med Coll, Trop Dis & Image Diag Dept, Botucatu, Brazil

[2] Sao Paulo State Univ UNESP, Anim Prod & Prevent Vet Med Dept FMVZ, Botucatu, Brazil

[3] Rural Fed Univ Pernambuco UFRPE, Dept Vet Med, Recife, Brazil

[4] Sao Paulo State Agcy Agribusiness Technol APTA SAA, Bauru, SP, Brazil

[5] Adolfo Lutz Inst, Bauru, SP, Brazil

[6] Biol Inst, Aracatuba, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2024年 / 409卷

基金：

巴西圣保罗研究基金会;

关键词：

Detection; Milk; Toxoplasmosis; Zoonosis; Bulk tank milk; IDENTIFICATION; DNA; ANTIBODIES; SAMPLES; GOATS; ELISA; SHEEP; PCR;

D O I：

10.1016/j.ijfoodmicro.2023.110466

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study aimed to research Toxoplasma gondii DNA in 102 samples of raw bovine milk from expansion tanks, in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil. For this, polymerase chain reaction (PCR) was performed with the primers TOX4/TOX5 for cPCR (conventional PCR), TgNP1/TgNP2 gene for nested PCR and the Tg18s58F/Tg18s348R for nested PCR. It was possible to detect T. gondii DNA in 18 (17.65 %) milk samples from the 102 tanks, corresponding to 4.90 % for TOX4/TOX5 primers, 12.74 % for TgNP1/TgNP2 gene and 0.98 % for Tg18s58F/Tg18s348R gene. The results showed that the TgNP1 and TgNP2 genes were more efficient to detect T. gondii DNA, and also indicated the importance of raw bovine milk as a source of human infections caused by this protozoan, being a public health problem. It is important to continue studies involving T. gondii from bovine milk considering the need for proper pasteurization, and for better comprehension regarding the epidemiology of this protozoan.

引用

页数：7

共 37 条

[1] Use of PCR to determine Toxoplasma gondii in milk samples from camels (Camelus dromedarius), cattle (Bos taurus) and buffalos (Bubalus bubalis) in East Azarbaijan province, Iran
Aghdam, Somayyeh Asiyabi
Hajipour, Nasser
Moosavy, Mir-Hassan
[J]. VETERINARY MEDICINE AND SCIENCE, 2023, 9 (01) : 400 - 404
[2] Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in Sao Paulo, Brazil
Bertolini, Amanda Bezerra
Orsi Dutra, Marcelo Augusto
Guiraldi, Livia Maisa
dos Santos, Wesley Jose
Merino de Medeiros, Maria Izabel
Lucheis, Simone Baldini
[J]. SEMINA-CIENCIAS AGRARIAS, 2019, 40 (02): : 723 - 730
[3] Detection of Toxoplasma gondii in the milk of naturally infected goats in the Northeast of Brazil
Bezerra, M. J. G.
Kim, P. C. P.
Moraes, E. P. B. X.
Sa, S. G.
Albuquerque, P. P. F.
Silva, J. G.
Alves, B. H. L. S.
Mota, R. A.
[J]. TRANSBOUNDARY AND EMERGING DISEASES, 2015, 62 (04) : 421 - 424
[4] Detection of Toxoplasma gondii DNA in the milk of naturally infected ewes
Camossi, L. G.
Greca-Junior, H.
Correa, A. P. F. L.
Richini-Pereira, V. B.
Silva, R. C.
Da Silva, A. V.
Langoni, H.
[J]. VETERINARY PARASITOLOGY, 2011, 177 (3-4) : 256 - 261
[5] Congenital Transmission of Toxoplasma gondii After Experimental Reinfection With Brazilian Typical Strains in Chronically Infected Sheep
Chiebao, Daniela Pontes
Pena, Hilda Fatima
Passarelli, Danielle
Santin, Thiago
Pulz, Lidia Hildebrand
Strefezzi, Ricardo Francisco
Seva, Anaia Paixao
Martins, Camila Marinelli
Lopes, Estela Gallucci
Hildebrand Grisi Filho, Jose Henrique
Gennari, Solange Maria
Soares, Rodrigo Martins
[J]. FRONTIERS IN VETERINARY SCIENCE, 2019, 6
[6] Presence of Pathogenic Rickettsiae and Protozoan in Samples of Raw Milk from Cows, Goats, and Sheep
Cisak, Ewa
Zajac, Violetta
Sroka, Jacek
Sawczyn, Anna
Kloc, Anna
Dutkiewicz, Jacek
Wojcik-Fatla, Angelina
[J]. FOODBORNE PATHOGENS AND DISEASE, 2017, 14 (04) : 189 - 194
[7] Gomes DFC, 2020, REV BRAS PARASITOL V, V29, DOI [10.1590/S1984-29612019106, 10.1590/s1984-29612019106]
[8] Artisan fresh cheese from raw cow's milk as a possible route of transmission in a toxoplasmosis outbreak, in Brazil
da Costa, Mary Alexandra
Pinto-Ferreira, Fernanda
Amorim de Almeida, Robelia Ponde
Cardoso Martins, Felippe Danyel
Pires, Adriene Lino
Mareze, Marcelle
Mitsuka-Bregano, Regina
Freire, Roberta Lemos
da Rocha Moreira, Rosalynd Vinicios
Borges, Juliene Meira
Navarro, Italmar Teodorico
[J]. ZOONOSES AND PUBLIC HEALTH, 2020, 67 (02) : 122 - 129
[9] da Cunha MDRD, 2006, BRAZ J MICROBIOL, V37, P70, DOI 10.1590/S1517-83822006000100013
[10] Detection of Toxoplasma gondii DNA in milk of dairy cows from southern Brazil
da Fonseca, Flavia Moreira
Sato, Ana Paula
Becker, Ana Paula Brenner Busch
Pinto, Gabriela Oliveira da Paz Augusto
de Souza, Giovana Scuissiatto
Perotta, Joao Henrique
de Barros Filho, Ivan Roque
Rodriguez, Maria Constanza
Locatelli-Dittrich, Rosangela
[J]. PARASITOLOGY INTERNATIONAL, 2023, 95

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