Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen

被引：2

作者：

Durasinovic, Tatjana ^{[1
]}

Lopandic, Zorana ^{[2
]}

Protic-Rosicb, Isidora ^{[2
]}

Nesic, Andrijana ^{[2
]}

Trbojevic-Ivic, Jovana ^{[3
]}

Jappe, Uta ^{[4
,5
]}

Gavrovic-Jankulovic, Marija ^{[2
]}

机构：

[1] Mil Med Acad, Inst Med Biochem, Belgrade 11000, Serbia

[2] Univ Belgrade, Fac Chem, Belgrade 11000, Serbia

[3] Belgrade Ltd, Fac Chem, Innovat Ctr, Belgrade 11000, Serbia

[4] Airway Res Ctr North ARCN, German Ctr Lung Res DZL, Res Ctr Borstel, Div Clin & Mol Allergol,Prior Area Asthma & Allerg, Borstel, Germany

[5] Univ Lubeck, Dept Pneumol, Interdisciplinary Allergy Outpatient Clin, Lubeck, Germany

来源：

FOOD CHEMISTRY | 2024年 / 437卷

关键词：

Banana; Food allergy; Recombinant allergen; Cross-reactivity; Panallergen; Epitope mapping; ALLERGEN; PROTEIN; ANAPHYLAXIS; REACTIVITY;

D O I：

10.1016/j.foodchem.2023.137782

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Banana allergy is often associated with the pollen and latex allergies, which led us to the hypothesis that some yet unidentified banana allergen could provide a basis of the latex-pollen-fruit syndrome. S-adenosyl-L-homocysteine hydrolase (SAHH) was recently identified in the literature as a novel plant allergen. This study aimed to assess the allergenic potential of the naturally occurring banana SAHH (nSAHH) and its recombinant homolog pro-duced in E. coli (rSAHH). nSAHH showed IgE reactivity with a serum pool of twelve banana-allergic persons, while rSAHH displayed IgE reactivity in ten out of the twelve tested patients. Five linear B-cell epitopes were identified on the rSAHH surface, exhibiting >= 90 % sequence homology with relevant plant SAHH allergens. Our findings have elucidated SAHH as a novel plant panallergen, underlying the cross-reactivity between plant-derived food and respiratory allergens, confirming our initial hypothesis.

引用

页数：7