Hydrodynamic disintegration of thickened excess sludge and maize silage to intensify methane production: Energy effect and impact on microbial communities

被引:9
作者
Garlicka, Agnieszka [1 ]
Umiejewska, Katarzyna [2 ]
Nielsen, Per Halkjaer [3 ]
Muszynski, Adam [2 ]
机构
[1] Joint Stock Co, Res & New Technol Off, Municipal Water Supply & Sewerage Co Capital City, Warsaw, Poland
[2] Warsaw Univ Technol, Fac Bldg Serv Hydro & Environm Engn, Warsaw, Poland
[3] Aalborg Univ, Ctr Microbial Communities, Dept Chem & Biosci, Aalborg, Denmark
关键词
Co; -digestion; Mechanical pretreatment; Renewable energy; Microbial communities; Bioavailability; CIRCULAR ECONOMY; CO-DIGESTION; WASTE; EXTRAPOLATION; PRETREATMENT; RAREFACTION;
D O I
10.1016/j.biortech.2023.128829
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The aim of this project was to study the combination of two methods to increase methane production: feedstock pretreatment by hydrodynamic disintegration and co-digestion of maize silage (MS) with thickened excess sludge (TES). Disintegration of TES alone resulted in a 15% increase in specific methane production from 0.192 Nml/ gVS (TES + MS) to 0.220 Nml/gVS (pretreated TES + MS). The energy balance revealed additional energy (0.14 Wh) would cover only the energy expenditure for the mechanical pretreatment and would not allow for net energy profit. Identification of the methanogenic consortia by 16S rRNA gene amplicon sequencing revealed that Chloroflexi, Bacteroidota, Firmicutes, Proteobacteria and Actinobacteriota were five most abundant bacteria phyla, with Methanothrix and Methanolinea as the dominant methanogens. Principal component analysis did not show any effect of feedstock pretreatment on methanogenic consortia. Instead, the composition of inoculum was the decisive factor in shaping the microbial community structure.
引用
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页数:11
相关论文
共 47 条
[1]   Enhancement of anaerobic digestion efficiency of wastewater sludge and olive waste: Synergistic effect of co-digestion and ultrasonic/microwave sludge pre-treatment [J].
Alagoz, B. Aylin ;
Yenigun, Orhan ;
Erdincler, Aysen .
WASTE MANAGEMENT, 2015, 46 :182-188
[2]   Back to Basics - The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities [J].
Albertsen, Mads ;
Karst, Soren M. ;
Ziegler, Anja S. ;
Kirkegaard, Rasmus H. ;
Nielsen, Per H. .
PLOS ONE, 2015, 10 (07)
[3]   Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton [J].
Apprill, Amy ;
McNally, Sean ;
Parsons, Rachel ;
Weber, Laura .
AQUATIC MICROBIAL ECOLOGY, 2015, 75 (02) :129-137
[4]   A year of monitoring 20 mesophilic full-scale bioreactors reveals the existence of stable but different core microbiomes in bio-waste and wastewater anaerobic digestion systems [J].
Calusinska, Magdalena ;
Goux, Xavier ;
Fossepre, Marie ;
Muller, Emilie E. L. ;
Wilmes, Paul ;
Delfosse, Philippe .
BIOTECHNOLOGY FOR BIOFUELS, 2018, 11
[5]   Microbial management of anaerobic digestion: exploiting the microbiome-functionality nexus [J].
Carballa, Marta ;
Regueiro, Leticia ;
Lema, Juan M. .
CURRENT OPINION IN BIOTECHNOLOGY, 2015, 33 :103-111
[6]   Rarefaction and extrapolation with Hill numbers: a framework for sampling and estimation in species diversity studies [J].
Chao, Anne ;
Gotelli, Nicholas J. ;
Hsieh, T. C. ;
Sander, Elizabeth L. ;
Ma, K. H. ;
Colwell, Robert K. ;
Ellison, Aaron M. .
ECOLOGICAL MONOGRAPHS, 2014, 84 (01) :45-67
[7]  
Clescerl L. S., 1998, STANDARD METHODS EXA
[8]  
Dane Otwarte, 2022, RAW MAT USED PRODUCT
[9]   MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants [J].
Dueholm, Morten Simonsen ;
Nierychlo, Marta ;
Andersen, Kasper Skytte ;
Rudkjobing, Vibeke ;
Knutsson, Simon ;
Albertsen, Mads ;
Nielsen, Per Halkjaer .
NATURE COMMUNICATIONS, 2022, 13 (01)
[10]   Generation of Comprehensive Ecosystem-Specific Reference Databases with Species-Level Resolution by High-Throughput Full-Length 16S rRNA Gene Sequencing and Automated Taxonomy Assignment (AutoTax) [J].
Dueholm, Morten Simonsen ;
Andersen, Kasper Skytte ;
McIlroy, Simon Jon ;
Kristensen, Jannie Munk ;
Yashiro, Erika ;
Karst, Soren Michael ;
Albertsen, Mads ;
Nielsen, Per Halkjaer .
MBIO, 2020, 11 (05) :1-14