3D imaging lipidometry in single cell by in-flow holographic tomography

被引：18

作者：

Pirone, Daniele ^{[1
,2
]}

Sirico, Daniele ^{[1
,3
]}

Miccio, Lisa ^{[1
]}

Bianco, Vittorio ^{[1
]}

Mugnano, Martina ^{[1
]}

del Giudice, Danila ^{[1
,4
]}

Pasquinelli, Gianandrea ^{[5
,6
]}

Valente, Sabrina ^{[5
]}

Lemma, Silvia ^{[7
,8
,9
]}

Iommarini, Luisa ^{[9
,10
]}

Kurelac, Ivana ^{[7
,8
,9
]}

Memmolo, Pasquale ^{[1
]}

Ferraro, Pietro ^{[1
]}

机构：

[1] Inst Appl Sci & Intelligent Syst E Caianiello, CNR ISASI, Via Campi Flegrei 34, I-80078 Naples, Italy

[2] Univ Naples Federico II, Dept Elect Engn & Informat Technol, Via Claudio 21, I-80125 Naples, Italy

[3] Univ Naples Federico II, Dept Chem & Mat & Prod Engn, Piazzale Tecchio 80, I-80125 Naples, Italy

[4] Univ Campania Luigi Vanvitelli, Dept Math & Phys, I-81100 Caserta, Italy

[5] Univ Bologna, Dept Expt Diagnost & Specialty Med DIMES, Biotechnol & Methods Lab Med, I-40126 Bologna, Italy

[6] Univ Bologna, Subcellular Nephrovasc Diagnost Program, Pathol Unit S Orsola IRCCS, I-40126 Bologna, Italy

[7] Univ Bologna, Dept Med & Surg Sci DIMEC, Unit Med Genet, Via Massarenti 9, I-40138 Bologna, Italy

[8] Univ Bologna, Study & Res Ctr Gynecol Neoplasias, Dept Med & Surg Sci DIMEC, Via Massarenti 9, I-40138 Bologna, Italy

[9] Univ Bologna, Ctr Appl Biomed Res CRBA, I-40138 Bologna, Italy

[10] Univ Bologna, Dept Pharm & Biotechnol FABIT, I-40126 Bologna, Italy

来源：

OPTO-ELECTRONIC ADVANCES | 2023年 / 6卷 / 01期

关键词：

lipid droplets; label -free phase -contrast imaging; in -flow tomography; 3D imaging; Introduction; LIPID DROPLETS; MICROSCOPY;

D O I：

10.29026/oea.2023.220048

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets (LDs) in several regulatory mechanisms in living cells. LDs are dynamic organelles and therefore their complete characterization in terms of number, size, spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs. Until now, fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity. However, such methods generally only provide 2D assays and partial measurements. Furthermore, both can be destructive and with low productivity, thus limiting analysis of large cell numbers in a sample. Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer, by using ovarian cancer cells and monocyte cell lines as models. A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported. The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.

引用

页数：16

共 62 条

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