Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure

被引：2

作者：

Egorov, V. V. ^{[1
,2
,3
,4
]}

Shvetsov, A. V. ^{[1
,3
,5
]}

Pichkur, E. B. ^{[3
]}

Shaldzhyan, A. A. ^{[2
]}

Zabrodskaya, Ya. A. ^{[2
,5
]}

Vinogradova, D. S. ^{[1
]}

Nekrasov, P. A. ^{[2
]}

Gorshkov, A. N. ^{[2
]}

Garmay, Yu. P. ^{[1
]}

Kovaleva, A. A. ^{[2
]}

Stepanova, L. A. ^{[2
]}

Tsybalova, L. M. ^{[2
]}

Shtam, T. A. ^{[1
,3
]}

Myasnikov, A. G. ^{[1
,3
]}

Konevega, A. L. ^{[1
,3
,5
]}

机构：

[1] Kurchatov Inst, Petersburg Nucl Phys Inst B P Konstantinov, Konstantinov Natl Res Ctr, Orlova Roscha 1, Gatchina 188300, Russia

[2] Russian Minist Hlth, Smorodintsev Res Inst Influenza, Prof Popov St 15-17, St Petersburg 197376, Russia

[3] Kurchatov Inst, Natl Res Ctr, Akademika Kurchatova pl 1, Moscow 123182, Russia

[4] Inst Expt Med, Academika Pavlova 12, St Petersburg 197376, Russia

[5] Peter Great St Petersburg Polytech Univ, Politehnicheskaya 29, St Petersburg, Russia

来源：

BIOPHYSICAL CHEMISTRY | 2023年 / 293卷

基金：

俄罗斯科学基金会;

关键词：

Virus -like particles; Hepatitis B virus core antigen; Influenza virus; Structural analysis; Cryo-electron microscopy; Molecular modeling; THIOFLAVINE-T INTERACTION; ANTIGENS;

D O I：

10.1016/j.bpc.2022.106943

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 angstrom (3.13 angstrom for particles of T = 4 symmetry, 3.7 angstrom for particles of T = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.

引用

页数：7

共 22 条

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