Electrophoretic Protein Deposition as a Tool for In Situ Co-Crosslinking Enzyme Immobilization: An Electrochemical/Quartz Crystal Microbalance Study

被引:1
|
作者
Guerrieri, Antonio [1 ]
Ciriello, Rosanna [1 ]
Acquavia, Maria Assunta [1 ]
Bianco, Giuliana [1 ]
Di Capua, Angela [1 ]
机构
[1] Univ Basilicata, Dipartimento Sci, Via Dellateneo Lucano 10, I-85100 Potenza, Italy
来源
APPLIED SCIENCES-BASEL | 2024年 / 14卷 / 01期
关键词
electrophoretic protein deposition; enzyme immobilization; crosslinking; glucose oxidase; bovine serum albumin; electroanalytical chemistry; electrochemical quartz crystal microbalance; OXIDASE/OVEROXIDIZED POLYPYRROLE BILAYER; GLUCOSE-OXIDASE; AMPEROMETRIC BIOSENSOR; L-LYSINE; PLATINUM-ELECTRODE; ACETYLCHOLINE; FABRICATION; CHOLINE; SENSOR; FILMS;
D O I
10.3390/app14010212
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Electrophoretic deposition is a powerful tool for depositing materials onto a substrate by using an electric field; its application in biotechnological areas, namely, electrophoretic protein deposition (EPD), is the most promising for, e.g., fabricating novel amperometric biosensors. Unfortunately, EPD suffers from several drawbacks due to coupled parasite electrochemical processes damaging the deposit; moreover, the nature of the deposition process, the deposit, and its stability are still controversial and unknown. The present research presents a deep investigation of the EPD processes conducted by using several electroanalytical techniques and an electrochemical quartz crystal microbalance (EQCM); notably, EPD was used here as a novel tool for performing an electrophoretically assisted, classical enzyme immobilization technique like co-crosslinking, thus permitting the immobilization of the desired protein in situ, i.e., exclusively onto the deposition electrode. An electrochemical study permitted the acquisition of useful insights about electrophoresis processes as well as solvent discharge and gas evolution at the deposition electrode; further, the use of appropriate current or potential pulse sequences, as investigated and improved in this study, together with fine-tuned chemical conditions, allowed the optimization of this novel EPD approach. Moreover, an EQCM study gave useful insights into the kinetics of the process, permitting a quantitative estimate of the deposit.
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页数:15
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