Boron-doped diamond nanosheet volume-enriched screen-printed carbon electrodes: a platform for electroanalytical and impedimetric biosensor applications

被引:3
作者
Ficek, Mateusz [1 ]
Cieslik, Mateusz [1 ,2 ]
Janik, Monika [1 ,3 ]
Brodowski, Mateusz [1 ]
Sawczak, Miroslaw [4 ]
Bogdanowicz, Robert [1 ]
Ryl, Jacek [1 ]
机构
[1] Gdansk Univ Technol, Narutowicza 11-12, PL-80233 Gdansk, Poland
[2] Univ Gdansk, Dept Analyt Chem, Fac Chem, Wita Stwosza 63, PL-80308 Gdansk, Poland
[3] Warsaw Univ Technol, Inst Microelect & Optoelect, Koszykowa 75, Warsaw, Poland
[4] Polish Acad Sci, Szewalski Inst Fluid Flow Machinery, Fiszera 14, PL-80231 Gdansk, Poland
关键词
Boron-doped diamond; Screen-printed electrodes; Electroanalysis; Pathogen detection; Haemophilus influenzae; NONTYPABLE HAEMOPHILUS-INFLUENZAE; ELECTROCHEMICAL PERFORMANCE; PROTEIN-D; POWDER; COMPOSITES; BINDING; GROWTH;
D O I
10.1007/s00604-023-05991-w
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper focuses on the development of a novel electrode based on boron-doped diamond nanosheet full-volume-enriched screen-printed carbon electrodes (BDDPE) for use as an impedimetric biosensor. Impedimetric biosensors offer high sensitivity and selectivity for virus detection, but their use as point-of-care devices is limited by the complexity of nanomaterials' architecture and the receptor immobilisation procedures. The study presents a two-step modification process involving the electroreduction of diazonium salt at the BDDPE and the immobilisation of antibodies using zero-length cross-linkers for a selective impedimetric biosensor of Haemophilus influenzae (Hi). The incorporation of diamond nanosheets into BDDPE leads to enhanced charge transfer and electrochemical behaviour, demonstrating greatly improved electrochemically active surface area compared with unmodified screen-printed electrodes (by 44% and 10% on average for [Ru(NH3)(6)]Cl-2 and K-3[Fe(CN)(6)], respectively). The presented sensing system shows high specificity towards protein D in Hi bacteria, as confirmed by negative controls against potential interference from other pathogens, with an estimated tolerance limit for interference under 12%. The Hi limit of detection by electrochemical impedance spectroscopy was 1 CFU/mL (measured at - 0.13 V vs BDDPE pseudo-reference), which was achieved in under 10 min, including 5 min sample incubation in the presence of the analyte.
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页数:14
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