SHR and SCR coordinate root patterning and growth early in the cell cycle

被引:16
作者
Winter, Cara M. [1 ,2 ]
Szekely, Pablo [1 ,2 ]
Popov, Vladimir [1 ]
Belcher, Heather [1 ]
Carter, Raina [1 ]
Jones, Matthew [3 ]
Fraser, Scott E. [3 ]
Truong, Thai V. [3 ]
Benfey, Philip N. [1 ,2 ]
机构
[1] Duke Univ, Dept Biol, Durham, NC 27708 USA
[2] Duke Univ, Howard Hughes Med Inst, Durham, NC 27708 USA
[3] Univ Southern Calif, Bridge Inst, Translat Imaging Ctr, Los Angeles, CA USA
基金
美国国家卫生研究院;
关键词
RADIAL ORGANIZATION; ARABIDOPSIS ROOT; PROTEIN; DIVISION; ORIENTATION; ACTIVATION; DROSOPHILA; MOVEMENT; CHOICE; SWITCH;
D O I
10.1038/s41586-023-06971-z
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Precise control of cell division is essential for proper patterning and growth during the development of multicellular organisms. Coordination of formative divisions that generate new tissue patterns with proliferative divisions that promote growth is poorly understood. SHORTROOT (SHR) and SCARECROW (SCR) are transcription factors that are required for formative divisions in the stem cell niche of Arabidopsis roots1,2. Here we show that levels of SHR and SCR early in the cell cycle determine the orientation of the division plane, resulting in either formative or proliferative cell division. We used 4D quantitative, long-term and frequent (every 15 min for up to 48 h) light sheet and confocal microscopy to probe the dynamics of SHR and SCR in tandem within single cells of living roots. Directly controlling their dynamics with an SHR induction system enabled us to challenge an existing bistable model3 of the SHR-SCR gene-regulatory network and to identify key features that are essential for rescue of formative divisions in shr mutants. SHR and SCR kinetics do not align with the expected behaviour of a bistable system, and only low transient levels, present early in the cell cycle, are required for formative divisions. These results reveal an uncharacterized mechanism by which developmental regulators directly coordinate patterning and growth. Quantitative time-resolved microscopy analysis of SHR and SCR dynamics in single cells of living Arabidopsis roots shows that these transcription factors coordinate formative and proliferative cell divisions early in the cell cycle.
引用
收藏
页码:611 / 616
页数:18
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