SRSF1 is essential for primary follicle development by regulating granulosa cell survival via mRNA alternative splicing

被引：4

作者：

Yao, Xiaohong ^{[1
]}

Wang, Chaofan ^{[1
]}

Yu, Weiran ^{[1
]}

Sun, Longjie ^{[1
]}

Lv, Zheng ^{[1
]}

Xie, Xiaomei ^{[1
]}

Tian, Shuang ^{[1
]}

Yan, Lu ^{[1
]}

Zhang, Hua ^{[1
]}

Liu, Jiali ^{[1
]}

机构：

[1] China Agr Univ, Coll Biol Sci, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2023年 / 80卷 / 11期

关键词：

SRSF1; Granulosa cell; Alternative splicing; DNA damage; BINDING; PROLIFERATION; REPLICATION; EXPRESSION; MATURATION; FACTOR-1; OOCYTE; OVARY; USP3;

D O I：

10.1007/s00018-023-04979-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Granulosa cell abnormalities are characteristics of premature ovarian insufficiency (POI). Abnormal expression of serine/arginine-rich splicing factor 1 (SRSF1) can cause various diseases, but the role of SRSF1 in mouse granulosa cells remains largely unclear. In this study, we found that SRSF1 was expressed in the nuclei of both mouse oocytes and granulosa cells. The specific knockout of Srsf1 in granulosa cells led to follicular development inhibition, decreased granulosa cell proliferation, and increased apoptosis. Gene Ontology (GO) analysis of RNA-seq results revealed abnormal expression of genes involved in DNA repair, cell killing and other signalling pathways. Alternative splicing (AS) analysis showed that SRSF1 affected DNA damage in granulosa cells by regulating genes related to DNA repair. In summary, SRSF1 in granulosa cells controls follicular development by regulating AS of genes associated with DNA repair, thereby affecting female reproduction.

引用

页数：11

共 38 条

[1] REGULATION OF MEIOTIC MATURATION IN THE MAMMALIAN OOCYTE - INTERPLAY BETWEEN EXOGENOUS CUES AND THE MICROTUBULE CYTOSKELETON [J].

ALBERTINI, DF .

BIOESSAYS, 1992, 14 (02) :97-103

[2] SRSF1-Regulated Alternative Splicing in Breast Cancer [J].

Anczukow, Olga ;

Akerman, Martin ;

Clery, Antoine ;

Wu, Jie ;

Shen, Chen ;

Shirole, Nitin H. ;

Raimer, Amanda ;

Sun, Shuying ;

Jensen, Mads A. ;

Hua, Yimin ;

Allain, Frederic H. -T. ;

Krainer, Adrian R. .

MOLECULAR CELL, 2015, 60 (01) :105-117

[3] The splicing factor SRSF1 regulates apoptosis and proliferation to promote mammary epithelial cell transformation [J].

Anczukow, Olga ;

Rosenberg, Avi Z. ;

Akerman, Martin ;

Das, Shipra ;

Zhan, Lixing ;

Karni, Rotem ;

Muthuswamy, Senthil K. ;

Krainer, Adrian R. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2012, 19 (02) :220-228

[4] HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes [J].

Bekker-Jensen, Simon ;

Danielsen, Jannie Rendtlew ;

Fugger, Kasper ;

Gromova, Irina ;

Nerstedt, Annika ;

Bartek, Jiri ;

Lukas, Jiri ;

Mailand, Niels .

NATURE CELL BIOLOGY, 2010, 12 (01) :80-U209

[5] Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence [J].

Carabatsos, MJ ;

Sellitto, C ;

Goodenough, DA ;

Albertini, DF .

DEVELOPMENTAL BIOLOGY, 2000, 226 (02) :167-179

[6] The SRSF1 linker induces semi-conservative ESE binding by cooperating with the RRMs [J].

Cho, Suhyung ;

Hoang, Amy ;

Chakrabarti, Sutapa ;

Nhat Huynh ;

Huang, De-Bin ;

Ghosh, Gourisankar .

NUCLEIC ACIDS RESEARCH, 2011, 39 (21) :9413-9421

[7] Interaction between the RNA binding domains of Ser-Arg splicing factor 1 and U1-70K snRNP protein determines early spliceosome assembly [J].

Cho, Suhyung ;

Hoang, Amy ;

Sinha, Rahul ;

Zhong, Xiang-Yang ;

Fu, Xiang-Dong ;

Krainer, Adrian R. ;

Ghosh, Gourisankar .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (20) :8233-8238

[8] Premature Ovarian Insufficiency: Past, Present, and Future [J].

Chon, Seung Joo ;

Umair, Zobia ;

Yoon, Mee-Sup .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2021, 9

[9] Emerging Functions of SRSF1, Splicing Factor and Oncoprotein, in RNA Metabolism and Cancer [J].

Das, Shipra ;

Krainer, Adrian R. .

MOLECULAR CANCER RESEARCH, 2014, 12 (09) :1195-1204

[10] The Mammalian Ovary from Genesis to Revelation [J].

Edson, Mark A. ;

Nagaraja, Ankur K. ;

Matzuk, Martin M. .

ENDOCRINE REVIEWS, 2009, 30 (06) :624-712

← 1 2 3 4 →