Isolation and characterization of microcrystalline cellulose from an agro-waste tamarind (Tamarindus indica) seeds and its suitability investigation for biofilm formulation

The exploration of potential bio-fillers for bio-film application is a promising approach to ensure biodegradable, eco-friendly, good-quality materials with high-performance applications. This is a comprehensive study executed to establish the utility of an agro-waste Tamarindus indica seeds for microcrystalline cellulose production and to assess its feasibility for biofilm fabrication. The extraction was carried out through consecutive chemicalmediated alkalization, acid hydrolysis and bleaching. The isolated microcrystalline cellulose from Tamarindus indica seeds (TSMCC) was characterized through chemical, thermal and morphological characterization to validate the cellulose contribution, thermal resistance, and compatibility of the material. The physical parameters as density and yield percentage were assessed to evaluate its light-weight utility and economic productivity. These examinations revealed that TSMCC has good specific properties such as high cellulose content (90.57 %), average density (1.561 g/cm3), feasible average roughness (12.161 nm), desired particle size (60.40 +/- 21.10 mu m), good crystallinity (CI-77.6 %) and thermal stability (up to 230 degrees C); which are worthwhile to consider TSMCC for bio-film formulation. Subsequently, bio-films were formulated by reinforcing TSMCC in polylactic acid (PLA) matrix and the mechanical properties of the bio-films were then studied to establish the efficacy of TSMCC. It is revealed that the properties of pure PLA film increased after being incorporated with TSMCC, where 5 %TSMCC addition showed greater impact on crystalline index (26.16 % to 39.62 %), thermal stability (333oc to 389 degrees C), tensile strength (36.11 +/- 2.90 MPa to 40.22 +/- 3.22 MPa) and modulus (2.62 +/- 0.55GPa to 4.15 +/- 0.53GPa). In light of all promising features, 5 % TSMCC is recommended as a potential filler reinforcement for the groundwork of good quality bio-films for active packaging applications in future.