The glycoside hydrolase 28 member VdEPG1 is a virulence factor of Verticillium dahliae and interacts with the jasmonic acid pathway-related gene GhOPR9

被引：12

作者：

Liu, Shichao ^{[1
,2
]}

Liu, Ruibing ^{[1
,2
]}

Lv, Junyuan ^{[1
]}

Feng, Zili ^{[1
]}

Wei, Feng ^{[1
,3
]}

Zhao, Lihong ^{[1
]}

Zhang, Yalin ^{[1
]}

Zhu, Heqin ^{[1
,3
,4
]}

Feng, Hongjie ^{[1
,3
,4
]}

机构：

[1] Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang, Henan, Peoples R China

[2] Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning, Hainan, Peoples R China

[3] Chinese Acad Agr Sci, Western Agr Res Ctr, Changji, Xinjiang, Peoples R China

[4] Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Henan, Peoples R China

来源：

MOLECULAR PLANT PATHOLOGY | 2023年 / 24卷 / 10期

基金：

中国国家自然科学基金;

关键词：

cell death; cotton; glycoside hydrolase; jasmonic acid; knock out; Verticillium dahliae; WALL-DEGRADING ENZYMES; BOTRYTIS-CINEREA; MOLECULAR-PATTERNS; DEFENSE RESPONSES; PLANT IMMUNITY; PROTEIN; ENDOPOLYGALACTURONASE; PATHOGENICITY; EXPRESSION; WILT;

D O I：

10.1111/mpp.13366

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Glycoside hydrolase (GH) family members act as virulence factors and regulate plant immune responses during pathogen infection. Here, we characterized the GH28 family member endopolygalacturonase VdEPG1 in Verticillium dahliae. VdEPG1 acts as a virulence factor during V. dahliae infection. The expression level of VdEPG1 was greatly increased in V. dahliae inoculated on cotton roots. VdEPG1 suppressed VdNLP1-mediated cell death by modulating pathogenesis-related genes in Nicotiana benthamiana. Knocking out VdEPG1 led to a significant decrease in the pathogenicity of V. dahliae in cotton. The deletion strains were more susceptible to osmotic stress and the ability of V. dahliae to utilize carbon sources was deficient. In addition, the deletion strains lost the ability to penetrate cellophane membrane, with mycelia showing a disordered arrangement on the membrane, and spore development was affected. A jasmonic acid (JA) pathway-related gene, GhOPR9, was identified as interacting with VdEPG1 in the yeast two-hybrid system. The interaction was further confirmed by bimolecular fluorescence complementation and luciferase complementation imaging assays in N. benthamiana leaves. GhOPR9 plays a positive role in the resistance of cotton to V. dahliae by regulating JA biosynthesis. These results indicate that VdEPG1 may be able to regulate host immune responses as a virulence factor through modulating the GhOPR9-mediated JA biosynthesis.

引用

页码：1238 / 1255

页数：18