Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

被引：6

作者：

Gross, Tara ^{[1
,2
]}

Dieterle, Martin Philipp ^{[3
]}

Vach, Kirstin ^{[4
]}

Altenburger, Markus Joerg ^{[1
,2
]}

Hellwig, Elmar ^{[1
]}

Proksch, Susanne ^{[1
,2
,5
]}

机构：

[1] Albert Ludwigs Univ Freiburg, Univ Freiburg, Fac Med, Med Ctr,Ctr Dent Med,Dept Operat Dent & Periodonto, Hugstetter Str 55, D-79106 Freiburg, Germany

[2] Albert Ludwigs Univ Freiburg, Univ Freiburg, Fac Med, Med Ctr,GERN Res Ctr Tissue Replacement Regenerat, Engesserstr 4, D-79108 Freiburg, Germany

[3] Albert Ludwigs Univ Freiburg, Univ Freiburg, Fac Med, Ctr Dent Med,Div Oral Biotechnol,Med Ctr, Hugstetter Str 55, D-79106 Freiburg, Germany

[4] Albert Ludwigs Univ Freiburg, Univ Freiburg, Inst Med Biometry & Stat, Fac Med,Med Ctr, Stefan Meier Str 26, D-79104 Freiburg, Germany

[5] Friedrich Alexander Univ Erlangen Nurnberg FAU, Univ Hosp Erlangen, Dent Clin Operat Dent & Periodontol 1, Gluckstr 11, D-91054 Erlangen, Germany

来源：

BIOENGINEERING-BASEL | 2023年 / 10卷 / 03期

关键词：

tissue engineering; dental pulp; stem cells; elasticity; scaffold; cellular mechanotransduction; regeneration;

D O I：

10.3390/bioengineering10030323

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dental pulp regeneration strategies frequently result in hard tissue formation and pulp obliteration. The aim of this study was to investigate whether dental pulp stem cells (DPSCs) can be directed toward soft tissue differentiation by extracellular elasticity. STRO-1-positive human dental pulp cells were magnetically enriched and cultured on substrates with elasticities of 1.5, 15, and 28 kPa. The morphology of DPSCs was assessed visually. Proteins relevant in mechanobiology ACTB, ITGB1, FAK, p-FAK, TALIN, VINCULIN, PAXILLIN, ERK 1/2, and p-ERK 1/2 were detected by immunofluorescence imaging. Transcription of the pulp marker genes BMP2, BMP4, MMP2, MMP3, MMP13, FN1, and IGF2 as well as the cytokines ANGPT1, VEGF, CCL2, TGFB1, IL2, ANG, and CSF1 was determined using qPCR. A low stiffness, i.e., 1.5 kPa, resulted in a soft tissue-like phenotype and gene expression, whereas DPSCs on 28 kPa substrates exhibited a differentiation signature resembling hard tissues with a low cytokine expression. Conversely, the highest cytokine expression was observed in cells cultured on intermediate elasticity, i.e., 15 kPa, substrates possibly allowing the cells to act as "trophic mediators". Our observations highlight the impact of biophysical cues for DPSC fate and enable the design of scaffold materials for clinical pulp regeneration that prevent hard tissue formation.

引用

页数：18