Jasmine tea extract enhances human retinal pigment epithelial cells survival after UVB irradiation

被引：2

作者：

Shang, Hu ^{[1
]}

Guo, Yaling ^{[1
]}

Wu, Liangyu ^{[1
]}

Lin, Jinke ^{[1
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Hort, Fuzhou, Peoples R China

来源：

APPLIED BIOLOGICAL CHEMISTRY | 2023年 / 66卷 / 01期

关键词：

Jasmine tea extract; Retinal pigment epithelial cells; UVB-irradiation; Oxidative stress; Apoptosis; OXIDATIVE STRESS; DAMAGE; POLYPHENOLS; COMPONENTS; APOPTOSIS;

D O I：

10.1186/s13765-023-00779-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To examine the protecting effect of jasmine tea extract (JTE) against ultraviolet B (UVB) induced damage on human retinal pigment epithelial (RPE) cells, the RPE cells were subjected to UVB exposure and sequential JTE administration. The cell viability, intracellular reactive oxygen species (ROS), and apoptosis were determined by MTT, 2MODIFIER LETTER PRIME,7MODIFIER LETTER PRIME-dichlorodihydrofluorescein diacetate and flow cytometer assays, respectively. Further, the cells treated with UVB irradiation and sequential JTE administration were subjected to RNA-sequencing analysis in order to identify genes and pathways involved in the UVB-induced damage and JTE protecting mechanisms. The results showed that JTE effectively attenuated the UVB-induced cell injury by reducing the excessive intracellular ROS generation, and inhibiting the expression of apoptotic genes such as Bax, Caspase-3/9. This finding may offer a promising candidate for the prevention of UVB exposure related eye diseases.

引用

页数：14

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