The 1H, 15N and 13C resonance assignments of dengue virus capsid protein with the deletion of the intrinsically disordered N-terminal region

被引：0

作者：

Barbosa, Glauce M. ^{[1
]}

Morando, Maria A. ^{[1
,2
]}

Da Poian, Andrea T. ^{[1
]}

Almeida, Fabio C. L. ^{[1
,3
]}

机构：

[1] Fed Univ Rio De Janeiro UFRJ, Inst Med Biochem Leopoldo Meis IBqM, Rio De Janeiro, RJ, Brazil

[2] Fiocruz MS, Ctr Desenvolvimento Tecnol Saude, BR-21040361 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Natl Ctr Struct Biol & Bioimaging CENABIO, Rio De Janeiro, RJ, Brazil

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2023年 / 17卷 / 01期

关键词：

Dengue Virus; Capsid Protein; NMR; Capsid assembly; Intrinsically disordered region;

D O I：

10.1007/s12104-022-10115-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Dengue virus belongs to the Flaviviridae family, being responsible for an endemic arboviral disease in humans. It is an enveloped virus, whose genome is a positive-stranded RNA packaged by the capsid protein. Dengue virus capsid protein (DENVC) forms homodimers in solution organized in 4 alpha-helices and an intrinsically disordered N-terminal region. The N-terminal region is involved in the binding of membranous structures in host cells and in the recognition of nucleotides. Here we report the H-1, N-15 and C-13 resonance assignments of the DENVC with the deletion of the first 19 intrinsically disordered residues. The backbone chemical shift perturbations suggest changes in the alpha 1 and alpha 2 helices between full length and the truncated proteins.

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页码：23 / 26

页数：4

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