In-vivo exposure of a plant model organism for the assessment of the ability of PM samples to induce oxidative stress

被引：2

作者：

Vaccarella, Emanuele ^{[1
]}

Piacentini, Diego ^{[1
]}

Falasca, Giuseppina ^{[1
]}

Canepari, Silvia ^{[1
,2
]}

Massimi, Lorenzo ^{[1
,2
]}

机构：

[1] Sapienza Univ Rome, Dept Environm Biol, Ple Aldo Moro 5, I-00185 Rome, Italy

[2] CNR Inst Atmospher Pollut Res, Via Salaria,Km 29,300 Monterotondo St, I-00015 Rome, Italy

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2023年 / 900卷

关键词：

Arabidopsis thaliana; Oxidative potential; Oxidative stress; Living organism; Particulate matter; Superoxide anion; PARTICULATE MATTER PM2.5; CHEMICAL-COMPOSITION; SEASONAL-VARIATIONS; MASS CONCENTRATION; AIR-POLLUTION; TOXICITY; CADMIUM; URBAN; VITRO; MECHANISMS;

D O I：

10.1016/j.scitotenv.2023.165694

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study aims to propose an innovative, simple, rapid, and cost-effective method to study oxidative stress induced by PM through in-vivo exposure of the plant model organism Arabidopsis thaliana. A. thaliana seedlings were exposed to urban dust certified for its elemental content and to PM2.5 samples collected in an urban-industrial area of Northern Italy. An innovative technique for the detachment and suspension in water of the whole intact dust from membrane filters was applied to expose the model organism to both the soluble and insoluble fractions of PM2.5, which were analyzed for 34 elements by ICP-MS. Oxidative stress induced by PM on A. thaliana was assessed by light microscopic localization and UV-Vis spectrophotometric determination of su-peroxide anion (& BULL;O2 �) content on the exposed seedlings by using the nitro blue tetrazole (NBT) assay. The results showed a good efficiency and sensitivity of the method for PM mass concentrations >20 & mu;g m- 3 and an increase in & BULL;O2 � content in all exposed seedlings, which mainly depends on the concentration, chemical composition, and sources of the PM administered to the model organism. Particles released by biomass burning appeared to contribute more to the overall toxicity of PM. This method was found to be cost-effective and easy to apply to PM collected on membrane filters in intensive monitoring campaigns in order to obtain valuable information on the ability of PM to generate oxidative stress in living organisms.

引用

页数：12

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