Engineered DNA molecular machine for ultrasensitive detection of environmental lead pollution

被引：6
作者：
Tanga, Hehe [1 ]
Chang, Wei [1 ,2 ]
Xue, Huijie [1 ]
Xu, Changlu [3 ]
Li, Zhi [3 ]
Liu, Hao [4 ]
Xing, Chao [5 ]
Liu, Gang [6 ]
Liu, Xiaoyan [1 ]
Wang, Hua [1 ,2 ]
Wang, Jie [1 ,2 ]
机构：
[1] Anhui Med Univ, Sch Pharm, Inflammat & Immune Mediated Dis Lab Anhui Prov, Hefei 230032, Peoples R China
[2] Anhui Med Univ, Key Lab Environm Toxicol Anhui Higher Educ Inst, Sch Publ Hlth, Dept Toxicol, Hefei 230032, Peoples R China
[3] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA USA
[4] Bengbu Med Coll, Sch Pharm, Bengbu 233000, Peoples R China
[5] Minjiang Univ, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350000, Fujian, Peoples R China
[6] Author Bur Lake Chaohu, Environm Monitoring Stn, Chaohu 238000, Peoples R China
来源：
JOURNAL OF HAZARDOUS MATERIALS | 2023年 / 459卷
关键词：
Lead pollution; Molecular machine; DNAzyme; QUANTITATIVE DETECTION; DNAZYME; BIOSENSORS;
D O I：
10.1016/j.jhazmat.2023.132306
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Dynamic monitoring of environmental Pb2+ is of utmost importance for food safety and personal well-being. Herein, we report a novel, rapid, and practical fluorescence detection platform for Pb2+. The platform comprises two essential components: an engineered DNAzyme probe (EDP) and a responsive functionalized probe (RFP). The EDP demonstrates specific recognition of Pb2+ and the subsequent release of free DNA fragments. The released DNA fragments are then captured using the RFP to form DNA complexes, which undergo multiple cascade amplification reactions involving polymerases and nickases, resulting in the generation of a large
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页数：9
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