Fine genetic mapping confers a major gene controlling leaf shape variation in watermelon

被引:5
作者
Xu, Ming [1 ]
Gao, Meiling [1 ,2 ]
Amanullah, Sikandar [3 ]
Guo, Yu [1 ,2 ]
Bao, Xiuping [1 ]
Duan, Yaru [1 ]
Liu, Xiujie [4 ]
Liu, Jixiu [4 ]
Gao, Yue [4 ]
Luan, Feishi [3 ]
机构
[1] Qiqihar Univ, Coll Life Sci Agr & Forestry, Qiqihar 161006, Peoples R China
[2] Heilongjiang Prov Key Lab Resistance Gene Engn &, Qiqihar 161006, Peoples R China
[3] Northeast Agr Univ, Coll Hort & Landscape Architecture, Harbin 150030, Peoples R China
[4] Qiqihar Agr Technol Extens Ctr, Qiqihar 161006, Peoples R China
基金
中国国家自然科学基金;
关键词
Watermelon; Leaf shape; BSA-seq; GWAS; LMI1; gene; MARGIN DEVELOPMENT; EVOLUTION; GENOME; LEAVES; L; EXPRESSION; PLANTS;
D O I
10.1007/s10681-023-03222-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Watermelon is an important horticultural crop in the Cucurbitaceae family, which exhibits a remarkable diversity of vegetative phenotypes. In this experimental study, two contrasted watermelon lines "natural mutant line (DW-2) with non-lobed leaves and the wild-type line (DG-2) with lobed leaves" were crossed to derive the biparental F-2 mapping populations over two years. Genetic segregation analysis suggested that the non-lobed leaf (nll) phenotype is controlled by a single genetic locus with incomplete dominance. Bulk segregant sequencing analysis (BSA-seq) and preliminary linkage mapping with 183 F-2 individuals identified the nll locus in a 0.7 Mbp region on chromosome 4, which was also verified in a genome-wide association study (GWAS) of 144 watermelon accessions with leaf variations. Fine genetic mapping with a large F-2 population (n = 1069) and screened recombinants exposed the delimited candidate region of 98.23 kb with eight functionally annotated genes. The sequence analysis in this region suggested that Cla97C04G076510 is the most likely candidate gene for regulating the nll, which encodes a homeobox leucine zipper (HD-Zip) transcription factor (ClNll), a homolog of the LATE MERISTEM IDENTITY1 (LMI1) in Arabidopsis. A single nucleotide deletion in the second exon of nll was mainly responsible for the non-lobed leaf phenotype, which encoded a truncated protein. A genetic marker based on the 1-bp deletion showed co-segregation with leaf lobeness in the F-2 population, exhibiting validation in the natural GWAS panel of 144 watermelon accessions. The phylogenetic associations of ClNll protein sequences with 11 homologous sequences from eight other plant species revealed an identical conserved function. Further, the expression level of the Cla97C04G076510 gene was noticed to be significantly lower in the non-lobed leaf of the DW-2 mutant. The identified results provide valuable insights for understanding the genetic regulation of leaf shape variation in watermelon and would be helpful for marker-assisted breeding aimed at the development of improved cultivars.
引用
收藏
页数:15
相关论文
共 65 条
[1]   Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.) [J].
Amanullah, Sikandar ;
Li, Shenglong ;
Osae, Benjamin Agyei ;
Yang, Tiantian ;
Abbas, Farhat ;
Gao, Meiling ;
Wang, Xuezheng ;
Liu, Hongyu ;
Gao, Peng ;
Luan, Feishi .
FRONTIERS IN PLANT SCIENCE, 2023, 13
[2]   Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon [J].
Amanullah, Sikandar ;
Osae, Benjamin Agyei ;
Yang, Tiantian ;
Li, Shenglong ;
Abbas, Farhat ;
Liu, Shi ;
Liu, Shusen ;
Song, Zhengfeng ;
Wang, Xuezheng ;
Gao, Peng ;
Luan, Feishi .
FRONTIERS IN PLANT SCIENCE, 2022, 13
[3]   Genetic linkage mapping and QTLs identification for morphology and fruit quality related traits of melon by SNP based CAPS markers [J].
Amanullah, Sikandar ;
Gao, Peng ;
Osae, Benjamin Agyei ;
Saroj, Arvind ;
Yang, Tiantian ;
Liu, Shi ;
Weng, Yiqun ;
Luan, Feishi .
SCIENTIA HORTICULTURAE, 2021, 278
[4]   Detection of putative QTL regions associated with ovary traits in melon using SNP-CAPS markers [J].
Amanullah, Sikandar ;
Saroj, Arvind ;
Osae, Benjamin Agyei ;
Liu, Shi ;
Liu, Hongyu ;
Gao, Peng ;
Luan, Feishi .
SCIENTIA HORTICULTURAE, 2020, 270
[5]   Modifications to a LATE MERISTEM IDENTITY1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.) [J].
Andres, Ryan J. ;
Coneva, Viktoriya ;
Frank, Margaret H. ;
Tuttle, John R. ;
Samayoa, Luis Fernando ;
Han, Sang-Won ;
Kaur, Baljinder ;
Zhu, Linglong ;
Fang, Hui ;
Bowman, Daryl T. ;
Rojas-Pierce, Marcela ;
Haigler, Candace H. ;
Jones, Don C. ;
Holland, James B. ;
Chitwood, Daniel H. ;
Kuraparthy, Vasu .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (01) :E57-E66
[6]   Mapping and genomic targeting of the major leaf shape gene (L) in Upland cotton (Gossypium hirsutum L.) [J].
Andres, Ryan J. ;
Bowman, Daryl T. ;
Kaur, Baljinder ;
Kuraparthy, Vasu .
THEORETICAL AND APPLIED GENETICS, 2014, 127 (01) :167-177
[7]  
BakerBrosh KF, 1997, ECOLOGY, V78, P1250, DOI 10.1890/0012-9658(1997)078[1250:TESOLA]2.0.CO
[8]  
2
[9]   Model for the regulation of Arabidopsis thaliana leaf margin development [J].
Bilsborough, Gemma D. ;
Runions, Adam ;
Barkoulas, Michalis ;
Jenkins, Huw W. ;
Hasson, Alice ;
Galinha, Carla ;
Laufs, Patrick ;
Hay, Angela ;
Prusinkiewicz, Przemyslaw ;
Tsiantis, Miltos .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (08) :3424-3429
[10]  
Bo K., 2021, THEOR APPL GENET, V58, P1