Synthesis of glutamine-based green emitting carbon quantum dots as a fluorescent nanoprobe for the determination of iron (Fe3+) in Solanum tuberrosum (potato)

Herein, we reported the use of N-doped green-emitting carbon quantum dots (N-CQDs) as a fluorescent probe for determining of Fe3+ ions in Solanum tuberosum for the first time. The N- CQDs were synthesised through an efficient, one-step, and safe hydrothermal technique using citric acid as the carbon source and glutamine as a novel nitrogen source. The temporal evolution of the optical properties was investigated by varying the synthetic conditions with respect to temperature (160 degrees C, 180 degrees C, 200 degrees C, 220 degrees C and 240 degrees C) and citric acid: glutamine precursor ratio (1:1, 1:1.5, l.2,1:3 and 1:4). The N-CQDs was characterised using Fourier-Transform Infra-red Spectroscopy (FTIR) High-resolution transmission electron microscope (HRTEM), ultra-violet-visible spectroscopy (UV-vis) and X-Ray diffraction analysis (XRD) while its stability was evaluated in different media; NaCl, Roswell Park Memorial Institute (RPMI) and Phosphate Buffered Saline (PBS), and at different pHs. The N-CQDs displayed green (525 nm) emission and were spherical with an average particle diameter of 3.41 & PLUSMN; 0.76 nm. The FTIR indicated car-boxylic, amino, and hydroxyl functional groups. The as-synthesised N-CQDs were stable in NaCl (up to 1 M), RPMI, and PBS without any significant change in its fluorescent intensity. The pH evaluation showed pHs 6 and 7 as the optimum pHs, while the fluorometric analysis showed selectivity towards Fe 3+ in the presence and absence of interfering ions. The detection limit of 1.05 & mu;M was calculated, and the photoluminescence mechanism revealed static quenching. The as-synthesised N-CQDs was used as a fluorescent nanoprobe to determine the amount of Fe3+ in Solanum tuberosum (Potatoes) tubers. The result showed a high level of accuracy (92.13-96.20%) when compared with an established standard analytical procedure with excellent recoveries of 99.23-103.9%. We believe the as-synthesised N-CQDs can be utilised as a reliable and fast fluorescence nanoprobe for the determining of Fe3+ ions.