Observing single cells in whole organs with optical imaging

被引：8

作者：

Yang, Xiaoquan ^{[1
,2
]}

Jiang, Tao ^{[2
]}

Liu, Lirui ^{[1
]}

Zhao, Xiaojun ^{[1
]}

Yu, Ximiao ^{[1
]}

Yang, Minjun ^{[1
]}

Liu, Guangcai ^{[1
]}

Luo, Qingming ^{[3
]}

机构：

[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, MoE Key Lab Biomed Photon, R China, Wuhan 430074, Peoples R China

[2] HUST Suzhou Inst Brainsmat, JITRI, Suzhou 215123, Peoples R China

[3] Hainan Univ, Sch Biomed Engn, Haikou, Peoples R China

来源：

JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES | 2023年 / 16卷 / 01期

关键词：

Single cell observation; whole organ; optical imaging; STRUCTURED-ILLUMINATION MICROSCOPY; HIGH-RESOLUTION; OXIDATIVE-PHOSPHORYLATION; PHOTOACOUSTIC MICROSCOPY; FLUORESCENCE MICROSCOPY; MULTIPHOTON MICROSCOPY; EXCITATION MICROSCOPY; RESPIRATORY ENZYMES; RAMAN-SPECTROSCOPY; LIGHT-MICROSCOPY;

D O I：

10.1142/S1793545823300021

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals. Then, we describe optical imaging to achieve three-dimensional spatial discrimination for biological tissues. Due to the milky appearance of tissues, the spatial information blurred deep in the whole organ. Fortunately, strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.

引用

页数：26

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