UPLC-QTOF-MS with a chemical profiling approach for holistic quality evaluation between a material reference of Wen Dan decoction and its commercial preparations

被引:9
作者
Yang, Siyu [1 ,2 ]
Chen, Gan [1 ,2 ]
Yuan, Man [1 ,2 ]
Zou, Yan [3 ]
Zhang, Hongmei [1 ]
Xu, Hongxi [4 ]
机构
[1] Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China
[2] Shanghai Coll TCM New Drug Discovery, Engn Res Ctr, Shanghai 201203, Peoples R China
[3] Shineway Pharmaceut Grp Ltd, Hebei, Peoples R China
[4] Shanghai Univ Tradit Chinese Med, Shuguang Hosp, Shanghai 201203, Peoples R China
关键词
Wen Dan decoction; Material reference; Commercial preparations; UPLC-QTOF-MS; Holistic quality evaluation; FLIGHT MASS-SPECTROMETRY; RAPID CHARACTERIZATION; CONSTITUENTS; FINGERPRINT; AUTOPHAGY;
D O I
10.1186/s13020-023-00767-z
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background Wen Dan decoction (WDD) has been a famous classic formula for resolving phlegm since ancient times in China. Currently, there are many types of WDD commercial preparations produced through modern technology. However, it is not known whether the holistic quality of WDD commercial preparations is consistent with the traditional decocting method to exert its proper effects. Therefore, the WDD material reference was studied and prepared, which can represent the traditional Chinese formulation WDD.Methods A method based on UPLC-QTOF-MS was developed to evaluate the quality of WDD material reference and commercial prescriptions. At the same time, the multivariate statistical method was used to compare the differences between the material reference and the commercial prescription by principal component analysis (PCA) and heatmap. Finally, the UPLC-QTOF-MS method was established to quantitatively study 11 representative components, including naringin, hesperidin, neohesperidin, liquiritin, glycyrrhizic acid, adenosine, liquiritigenin, tangeretin, eriocitrin, naringenin and synephrine.Results A total of 107 compounds were identified in the WDD material reference by comparing the retention time and fragment ion characteristics, including 54 flavonoids, 14 triterpenes, 10 organic acids, 7 alkaloids, 7 coumarins and 15 other components. The samples were almost evenly split into two groups, indicating a difference in quality between the WDD material reference and its commercial preparations in multivariate statistical analysis. Eleven major components of linearity, precision, repeatability, stability and recovery rate met the requirements, which were clearly different in commercial preparations and WDD material references. In terms of the content of 11 components in the commercial preparation, only CP8 is close to the material reference, which is in agreement with the statistical analysis of the heatmap. The concentrations of naringin and neohesperidin from the WDD material reference were higher than those from the commercial preparations.Conclusions The quality evaluation method established in this study can be used to identify different sources of WDD but also proves that the WDD material reference contains higher naringin. Furthermore, this study confirmed that the preparation technology of WDD commercial prescriptions should be optimized on the basis of WDD material references, producing the closest possible clinical basis for the substance.
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页数:24
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共 54 条
  • [1] Chang Guan-Hua, 2021, Zhongguo Zhong Yao Za Zhi, V46, P1449, DOI 10.19540/j.cnki.cjcmm.20201225.301
  • [2] Identification and Analysis of Chemical Constituents and Rat Serum Metabolites in Gushuling Using UPLC-Q-TOF/MS Coupled with Novel Informatics UNIFI Platform
    Chang, Hong
    Lv, Shujie
    Yuan, Tengteng
    Wu, Huan
    Wang, Lei
    Sang, Ran
    Zhang, Caiyun
    Chen, Weidong
    [J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2021, 2021
  • [3] The Lipid-Modulating Effect of Tangeretin on the Inhibition of Angiopoietin-like 3 (ANGPTL3) Gene Expression through Regulation of LXRα Activation in Hepatic Cells
    Chen, Pei-Yi
    Chao, Tzu-Ya
    Hsu, Hao-Jen
    Wang, Chih-Yang
    Lin, Ching-Yen
    Gao, Wan-Yun
    Wu, Ming-Jiuan
    Yen, Jui-Hung
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (18)
  • [4] [陈佩东 Chen Peidong], 2014, [中成药, Chinese Traditional Patent Medicine], V36, P2115
  • [5] Therapeutic potential of naringin: an overview
    Chen, Rui
    Qi, Qiao-Ling
    Wang, Meng-Ting
    Li, Qi-Yan
    [J]. PHARMACEUTICAL BIOLOGY, 2016, 54 (12) : 3203 - 3210
  • [6] [崔美娜 Cui Meina], 2021, [中草药, Chinese Traditional and Herbal Drugs], V52, P7428
  • [7] Characterization and classification of seven Citrus herbs by liquid chromatography-quadrupole time-of-flight mass spectrometry and genetic algorithm optimized support vector machines
    Duan, Li
    Guo, Long
    Liu, Ke
    Liu, E. -Hu
    Li, Ping
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2014, 1339 : 118 - 127
  • [8] Qualitative and Quantitative Analysis of 24 Components in Jinlianhua Decoction by UPLC-MS/MS
    Fang, Mingyue
    Liu, Shuangyue
    Wang, Qingqing
    Gu, Xuan
    Ding, Pengmin
    Wang, Weihua
    Ding, Yi
    Liu, Junxiu
    Wang, Rufeng
    [J]. CHROMATOGRAPHIA, 2019, 82 (12) : 1801 - 1825
  • [9] Rapid characterization of the chemical constituents of Sijunzi decoction by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry
    Guan, Zhibo
    Wang, Miao
    Cai, Yi
    Yang, Hongmei
    Zhao, Min
    Zhao, Chunjie
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2018, 1086 : 11 - 22
  • [10] Hao Y.M., 2020, J CHIN MED MAT, V43, P354