Battery of In Vitro Bioassays: A Case Study for the Cost-Effective and Effect-Based Evaluation of Wastewater Effluent Quality

被引：3

作者：

Alygizakis, Nikiforos ^{[1
,2
]}

Ng, Kelsey ^{[2
,3
]}

Maragou, Niki ^{[1
]}

Alirai, Sylvana ^{[1
]}

Behnisch, Peter ^{[4
]}

Besselink, Harrie ^{[4
]}

Oswald, Peter ^{[2
]}

Cirka, Lubos ^{[2
,5
]}

Thomaidis, Nikolaos S. S. ^{[1
]}

Slobodnik, Jaroslav ^{[2
]}

机构：

[1] Natl & Kapodistrian Univ Athens, Dept Chem, Lab Analyt Chem, Panepistimiopolis Zografou, Athens 15771, Greece

[2] Environm Inst, Okruzna 784-42, Kos 97241, Slovakia

[3] Masaryk Univ, Fac Sci, RECETOX, Kamenice 753-5,Bldg D29, Brno 62500, Czech Republic

[4] BioDetect Syst b v, Sci Pk 406, NL-1098 XH Amsterdam, Netherlands

[5] Slovak Univ Technol STU, Inst Informat Engn, Fac Chem & Food Technol, Automation, Radlinskeho 9, Bratislava 81237, Slovakia

来源：

WATER | 2023年 / 15卷 / 04期

关键词：

Danube River Basin; wastewater treatment plants; organic micropollutants; effect-based trigger values; toxicity endpoints; endocrine-disrupting compounds; mixture toxicity; environmental risk assessment; risk management; drinking water; TREATMENT-PLANT EFFLUENTS; TRIGGER VALUES; MIXTURES; CONTAMINANTS; IMPACT;

D O I：

10.3390/w15040619

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Wastewater treatment plants (WWTPs) represent an important input of contaminants in the environment. Therefore, it is critical to continuously monitor the performance of WWTPs to take appropriate action and avoid an influx of contaminants in the environment. In this study, a battery of seven in vitro bioassays covering a selected spectrum of toxicity effects is proposed for quality control of wastewater effluents. The bioassays address mixture toxicity, which is the combined adverse effect of multiple contaminants and can act as an early warning system. The proposed battery was applied to samples from 11 WWTPs of representative technology from the Danube River Basin (DRB). The order of toxic effects in terms of extent of exceedance of effect-based trigger values (EBTs) was PAH (PAH activity) > PXR (xenobiotic metabolism) > ER alpha (estrogenic activity) > PPAR(gamma) > Nrf(2) (oxidative stress) > anti-AR > GR. A mitigation plan for WWTP operators based on EBT exceedance is proposed. This study demonstrates that the proposed effect-based monitoring battery is a complementary tool to the chemical analysis approach. A regular application of such time- and cost-effective bioanalytical tools in the WWTPs of the DRB is proposed to provide a 'safety net' for aquatic ecosystems.

引用

页数：11

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