The recovery of intestinal barrier function and changes in oral microbiota after radiation therapy injury

被引:6
作者
Wang, Kun [1 ]
Zhang, Jingjing [2 ]
Zhang, Yihao [1 ]
Xue, Junze [1 ]
Wang, He [1 ]
Tan, Xiaojie [1 ]
Jiao, Xuelong [1 ]
Jiang, Haitao [1 ]
机构
[1] Qingdao Univ, Dept Gastrointestinal Surg, Affiliated Hosp, Qingdao, Peoples R China
[2] Qingdao Univ, Dept Pathol, Affiliated Hosp, Qingdao, Peoples R China
关键词
16s rRNA gene sequencing; rectal cancer radiotherapy; oral flora; intestinal mucosal barrier; rectal cancer; TIGHT JUNCTION PERMEABILITY; INCREASE; CANCER;
D O I
10.3389/fcimb.2023.1288666
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Introduction Colorectal cancer (CRC) is the third most common malignant tumor, and neoadjuvant chemo-radiotherapy is usually recommended for advanced stage colorectal cancer. Radiotherapy can cause damage to intestinal mucosal barrier, which may be related to perioperative complications. Intestinal microbiota is one of the constituents of the intestinal mucosal biological barrier, and literature reports that patients with CRC have changes in corresponding oral microbiota. This study aims to analyze the levels of immunoglobulin SIgA, inflammatory factors, lymphocyte subsets quantity, and proportion in surgical specimens of intestinal mucosa at different time intervals after radiotherapy, in order to seek investigation for the optimal surgical time after radiotherapy and to provide evidence for finding probiotics or immunomodulators through high-throughput sequencing of bacterial 16s rRNA in patients' saliva microbiota. Ultimately, this may provide new ideas for reducing perioperative complications caused by radiotherapy-induced intestinal damage.Methods We selected intestinal mucosal tissue and saliva samples from over 40 patients in our center who did not undergo radiotherapy and underwent surgery at different time intervals after radiotherapy. Detection of SIgA was performed using ELISA assay. Western Blotting was used to detect IL-1 beta, IL-6, and IL-17 in the intestinal mucosal tissue. Flow cytometry was used to detect CD4 and CD8. And the microbial community changes in saliva samples were detected through 16s rRNA sequencing.Results After radiotherapy, changes in SIgA, various cytokines, CD4CD8 lymphocyte subsets, and oral microbiota in the intestinal mucosal tissue of rectal cancer patients may occur. Over time, this change may gradually recover.Discussion In colorectal cancer, oncological aspects often receive more attention, while studies focusing on the intestinal mucosal barrier are less common. This study aims to understand the repair mechanisms of the intestinal mucosal barrier and reduce complications arising from radiotherapy-induced damage. The relationship between oral microbiota and systemic diseases has gained interest in recent years. However, the literature on the oral microbiota after radiotherapy for rectal cancer remains scarce. This study addresses this gap by analysing changes in the salivary microbiota of rectal cancer patients before and after radiotherapy, shedding light on microbiota changes. It aims to lay the groundwork for identifying suitable probiotics or immunomodulators to alleviate perioperative complications and improve the prognosis of CRC.
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页数:10
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共 27 条
[21]   Differential regulation of interleukin 17 and interferon γ production in inflammatory bowel disease [J].
Rovedatti, L. ;
Kudo, T. ;
Biancheri, P. ;
Sarra, M. ;
Knowles, C. H. ;
Rampton, D. S. ;
Corazza, G. R. ;
Monteleone, G. ;
Di Sabatino, A. ;
MacDonald, T. T. .
GUT, 2009, 58 (12) :1629-1636
[22]  
Sémont A, 2006, ADV EXP MED BIOL, V585, P19
[23]   IgA synthesis: a form of functional immune adaptation extending beyond gut [J].
Sutherland, Duncan B. ;
Fagarasan, Sidonia .
CURRENT OPINION IN IMMUNOLOGY, 2012, 24 (03) :261-268
[24]   Regulation of intestinal epithelial permeability by tight junctions [J].
Suzuki, Takuya .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2013, 70 (04) :631-659
[25]   Systematic review: the role of the gut microbiota in chemotherapy- or radiation-induced gastrointestinal mucositis - current evidence and potential clinical applications [J].
Touchefeu, Y. ;
Montassier, E. ;
Nieman, K. ;
Gastinne, T. ;
Potel, G. ;
des Varannes, S. Bruley ;
Le Vacon, F. ;
de La Cochetiere, M. F. .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2014, 40 (05) :409-421
[26]   The efficacy of bone marrow mesenchymal stem cells on rat intestinal immune-function injured by ischemia/reperfusion [J].
Wang, He ;
Wang, Kun ;
Liu, Bo ;
Bian, Xiaoqian ;
Tan, Xiaojie ;
Jiang, Haitao .
HELIYON, 2023, 9 (05)
[27]   Radiation Therapy for Rectal Cancer: Executive Summary of an ASTRO Clinical Practice Guideline [J].
Wo, Jennifer Y. ;
Anker, Christopher J. ;
Ashman, Jonathan B. ;
Bhadkamkar, Nishin A. ;
Bradfield, Lisa ;
Chang, Daniel T. ;
Dorth, Jennifer ;
Garcia-Aguilar, Julio ;
Goff, David ;
Jacqmin, Dustin ;
Kelly, Patrick ;
Newman, Neil B. ;
Olsen, Jeffrey ;
Raldow, Ann C. ;
Ruiz-Garcia, Erika ;
Stitzenberg, Karyn B. ;
Thomas, Charles R., Jr. ;
Wu, Q. Jackie ;
Das, Prajnan .
PRACTICAL RADIATION ONCOLOGY, 2021, 11 (01) :13-25