Supplementing L-Citrulline Can Extend Lifespan in C. elegans and Attenuate the Development of Aging-Related Impairments of Glucose Tolerance and Intestinal Barrier in Mice

被引:3
作者
Rajcic, Dragana [1 ]
Kromm, Franziska [1 ]
Hernandez-Arriaga, Angelica [2 ]
Brandt, Annette [1 ]
Baumann, Anja [1 ]
Staltner, Raphaela [1 ]
Camarinha-Silva, Amelia [2 ]
Bergheim, Ina [1 ]
机构
[1] Univ Vienna, Dept Nutr Sci, Mol Nutr Sci, A-1090 Vienna, Austria
[2] Univ Hohenheim, Inst Anim Sci, D-70593 Stuttgart, Germany
基金
奥地利科学基金会;
关键词
microbiota; C; elegans; senescence; nitric oxide; lifespan; PLASMINOGEN-ACTIVATOR INHIBITOR-1; BINDING-PROTEIN; AGE; METABOLISM; MICROBIOTA; ENDOTOXIN;
D O I
10.3390/biom13111579
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
L-Citrulline (L-Cit) is discussed to possess a protective effect on intestinal barrier dysfunction but also to diminish aging-associated degenerative processes. Here, the effects of L-Cit on lifespan were assessed in C. elegans, while the effects of L-Cit on aging-associated decline were determined in C57BL/6J mice. For lifespan analysis, C. elegans were treated with +/- 5 mM L-Cit. Twelve-month-old male C57BL/6J mice (n = 8-10/group) fed a standard chow diet received drinking water +/- 2.5 g/kg/d L-Cit or 5 g/kg/d hydrolyzed soy protein (Iso-N-control) for 16 or 32 weeks. Additionally, 4-month-old C57BL/6J mice were treated accordingly for 8 weeks. Markers of senescence, glucose tolerance, intestinal barrier function, and intestinal microbiota composition were analyzed in mice. L-Cit treatment significantly extended the lifespan of C. elegans. The significant increase in markers of senescence and signs of impaired glucose tolerance found in 16- and 20-month-old control mice was attenuated in L-Cit-fed mice, which was associated with protection from intestinal barrier dysfunction and a decrease in NO2- levels in the small intestine, while no marked differences in intestinal microbiota composition were found when comparing age-matched groups. Our results suggest that pharmacological doses of L-Cit may have beneficial effects on lifespan in C. elegans and aging-associated decline in mice.
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页数:16
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