Metabolic fingerprinting by nuclear magnetic resonance of hepatocellular carcinoma cells during p53 reactivation-induced senescence

被引：1

作者：

Knopf, Philipp ^{[1
,2
]}

Pacheco-Torres, Jesus ^{[2
]}

Zizmare, Laimdota ^{[1
,3
]}

Mori, Noriko ^{[2
]}

Wildes, Flonne ^{[2
]}

Zhou, Benyuan ^{[1
]}

Krishnamachary, Balaji ^{[2
]}

Mironchik, Yelena ^{[2
]}

Kneilling, Manfred ^{[1
,3
,4
]}

Trautwein, Christoph ^{[1
,3
]}

Pichler, Bernd J. ^{[1
,3
,5
,6
]}

Bhujwalla, Zaver M. ^{[2
,7
,8
,9
]}

机构：

[1] Eberhard Karls Univ Tubingen, Werner Siemens Imaging Ctr, Dept Preclin Imaging & Radiopharm, Tubingen, Germany

[2] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Div Canc Imaging Res, Baltimore, MD USA

[3] Univ Tubingen, Cluster Excellence iFIT EXC2180 Image Guided & Fun, Tubingen, Germany

[4] Eberhard Karls Univ Tubingen, Dept Dermatol, Tubingen, Germany

[5] German Canc Consortium DKTK, Tubingen, Germany

[6] German Canc Res Ctr DKFZ Partner Site Tubingen, Tubingen, Germany

[7] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Sch Med, Baltimore, MD USA

[8] Johns Hopkins Univ, Sch Med, Dept Radiat Oncol & Mol Radiat Sci, Baltimore, MD USA

[9] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Div Canc Imaging Res, Baltimore, MD 21205 USA

来源：

NMR IN BIOMEDICINE | 2024年 / 37卷 / 09期

关键词：

cellular homeostasis; metabolomics; MR spectroscopy; p53; senescence; T-2; RELAXATION-TIMES; N-ACETYL ASPARTATE; HUMAN BRAIN; RAT-BRAIN; WHITE-MATTER; H-1-NMR SPECTROSCOPY; AGE-DEPENDENCE; TISSUE; CREATINE; SPECTRA;

D O I：

10.1002/nbm.5157

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Cellular senescence is characterized by stable cell cycle arrest. Senescent cells exhibit a senescence-associated secretory phenotype that can promote tumor progression. The aim of our study was to identify specific nuclear magnetic resonance (NMR) spectroscopy-based markers of cancer cell senescence. For metabolic studies, we employed murine liver carcinoma Harvey Rat Sarcoma Virus (H-Ras) cells, in which reactivation of p53 expression induces senescence. Senescent and nonsenescent cell extracts were subjected to high-resolution proton (1H)-NMR spectroscopy-based metabolomics, and dynamic metabolic changes during senescence were analyzed using a magnetic resonance spectroscopy (MRS)-compatible cell perfusion system. Additionally, the ability of intact senescent cells to degrade the extracellular matrix (ECM) was quantified in the cell perfusion system. Analysis of senescent H-Ras cell extracts revealed elevated sn-glycero-3-phosphocholine, myoinositol, taurine, and creatine levels, with decreases in glycine, o-phosphocholine, threonine, and valine. These metabolic findings were accompanied by a greater degradation index of the ECM in senescent H-Ras cells than in control H-Ras cells. MRS studies with the cell perfusion system revealed elevated creatine levels in senescent cells on Day 4, confirming the 1H-NMR results. These senescence-associated changes in metabolism and ECM degradation strongly impact growth and redox metabolism and reveal potential MRS signals for detecting senescent cancer cells in vivo. This study investigates the metabolic differences between senescent and control cells and their ability to degrade the extracellular matrix (ECM). Senescent H-Ras cell extracts exhibit elevated sn-glycero-3-phosphocholine, myo-inositol, taurine, and creatine levels with a decrease of glycine, o-phosphocholine, threonine, and valine. These metabolic findings were accompanied by a higher extracellular matrix (ECM) degradation index of senescent compared to control H-Ras cells. image

引用

页数：12

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