An endoplasmic reticulum-targeted ratiometric fluorescent probe for hypochlorite bioimaging based on aggregation-induced ratiometric emission

被引：11

作者：

Chang, Xiao-Chuang ^{[1
]}

Zhang, Ling ^{[1
]}

Liu, Bing-Jie ^{[1
]}

Lou, Yan-Tao ^{[1
]}

Xu, Wei-Fan ^{[1
]}

Li, Xin-Ru ^{[1
]}

Han, Xue-Feng ^{[2
]}

Wu, Wei-Na ^{[1
]}

Wang, Yuan ^{[1
]}

Xu, Zhi-Hong ^{[3
,4
]}

机构：

[1] Henan Polytech Univ, Henan Key Lab Coal Green Convers, Coll Chem & Chem Engn, Jiaozuo 454000, Peoples R China

[2] Henan Polytech Univ, Coll Safety Sci & Engn, Jiaozuo 454000, Peoples R China

[3] Xuchang Univ, Coll Chem & Mat Engn, Key Lab Chemo Biosensing & Detect, Xuchang 461000, Peoples R China

[4] Zhengzhou Univ, Coll Chem, Zhengzhou 450052, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 2023年 / 191卷

基金：

中国国家自然科学基金;

关键词：

Bioimaging; Endoplasmic reticulum-targeted; Fluorescent probe; Hypochlorite; Pyrene; ACID; CELLS;

D O I：

10.1016/j.microc.2023.108809

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel pyrene-based fluorescent probe was synthesized, with an aggregation-induced ratiometric emission (AIRE) at 525 nm in 99% H2O solution (containing 1% DMSO). This AIRE was quenched, and a new blue emission at 470 nm was detected after adding hypochlorite (ClO-), according to a fast (within 20 s) and sensitive (detection limit of 269 nM) ratiometric fluorescent response to ClO-. The sensing mechanism of the probe to ClO- was assessed by ESI-MS, transmission electron microscopy, time-resolved fluorescence measurements, and density functional theory. In addition, the probe is equipped with p-toluenesulfonamide as the endoplasmic reticulum (ER) localization group, and has been applied to the imaging of endogenous and exogenous ClO- in both living cells and zebrafish.

引用

页数：7

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