The Myb73-GDPD2-GA2ox1 transcriptional regulatory module confers phosphate deficiency tolerance in soybean

被引：24

作者：

Hu, Dandan ^{[1
]}

Cui, Ruifan ^{[1
]}

Wang, Ke ^{[1
]}

Yang, Yuming ^{[1
]}

Wang, Ruiyang ^{[1
]}

Zhu, Hongqing ^{[1
]}

He, Mengshi ^{[1
]}

Fan, Yukun ^{[1
]}

Wang, Le ^{[2
]}

Wang, Li ^{[3
]}

Chu, Shanshan ^{[1
]}

Zhang, Jinyu ^{[1
]}

Zhang, Shanshan ^{[1
]}

Yang, Yifei ^{[1
]}

Zhai, Xuhao ^{[1
]}

Lu, Haiyan ^{[1
]}

Zhang, Dandan ^{[4
]}

Wang, Jinshe ^{[5
]}

Kong, Fanjiang ^{[6
]}

Yu, Deyue ^{[3
]}

Zhang, Hengyou ^{[2
]}

Zhang, Dan ^{[1
]}

机构：

[1] Henan Agr Univ, Coll Agron, Collaborat Innovat Ctr Henan Grain Crops, Zhengzhou 450002, Peoples R China

[2] Northeast Inst Geog & Agroecol, Chinese Acad Sci, State Key Lab Black Soils Conservat & Utilizat, Key Lab Soybean Mol Design Breeding, Harbin 150081, Peoples R China

[3] Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

[4] Huazhong Agr Univ, Coll Life Sci & Technol, Ctr Integrat Biol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[5] Henan Acad Agr Sci, Zhengzhou Natl Subctr Soybean Improvement, Zhengzhou 450002, Peoples R China

[6] Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China

来源：

PLANT CELL | 2024年 / 36卷 / 06期

基金：

中国国家自然科学基金;

关键词：

QUANTITATIVE TRAIT LOCI; PHOSPHORUS UPTAKE; ROOT-GROWTH; EXPRESSION; STARVATION; GENES; RESPONSES; STRESS; FAMILY; AUXIN;

D O I：

10.1093/plcell/koae041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphorus is indispensable in agricultural production. An increasing food supply requires more efficient use of phosphate due to limited phosphate resources. However, how crops regulate phosphate efficiency remains largely unknown. Here, we identified a major quantitative trait locus, qPE19, that controls 7 low-phosphate (LP)-related traits in soybean (Glycine max) through linkage mapping and genome-wide association studies. We identified the gene responsible for qPE19 as GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE2 (GmGDPD2), and haplotype 5 represents the optimal allele favoring LP tolerance. Overexpression of GmGDPD2 significantly affects hormone signaling and improves root architecture, phosphate efficiency and yield-related traits; conversely, CRISPR/Cas9-edited plants show decreases in these traits. GmMyb73 negatively regulates GmGDPD2 by directly binding to its promoter; thus, GmMyb73 negatively regulates LP tolerance. GmGDPD2 physically interacts with GA 2-oxidase 1 (GmGA2ox1) in the plasma membrane, and overexpressing GmGA2ox1 enhances LP-associated traits, similar to GmGDPD2 overexpression. Analysis of double mutants for GmGDPD2 and GmGA2ox1 demonstrated that GmGDPD2 regulates LP tolerance likely by influencing auxin and gibberellin dose-associated cell division in the root. These results reveal a regulatory module that plays a major role in regulating LP tolerance in soybeans and is expected to be utilized to develop phosphate-efficient varieties to enhance soybean production, particularly in phosphate-deficient soils. The major quantitative trait locus gene GmGDPD2 controls root and phosphorus efficiency traits and interacts with Myb73 and GA2ox1 to enhance yield traits.

引用

页码：2176 / 2200

页数：25

共 106 条

[1] Metabolomics and Transcriptomics in Legumes Under Phosphate Deficiency in Relation to Nitrogen Fixation by Root Nodules [J].