Effect of Yeast Saccharomyces cerevisiae as a Probiotic on Diet Digestibility, Fermentative Metabolites, and Composition and Functional Potential of the Fecal Microbiota of Dogs Submitted to an Abrupt Dietary Change

被引:20
作者
Bastos, Tais Silvino [1 ]
Menezes Souza, Camilla Mariane [1 ]
Legendre, Heloise [2 ]
Richard, Nadege [2 ]
Pilla, Rachel [3 ]
Suchodolski, Jan S. [3 ]
de Oliveira, Simone Gisele [1 ]
Lesaux, Achraf Adib [2 ]
Felix, Ananda Portella [1 ]
机构
[1] Univ Fed Parana, Dept Anim Sci, 1540, BR-80035050 Curitiba, PR, Brazil
[2] Phileo Lesaffre, 137 Rue Gabriel Peri, F-59700 Marcq En Baroeul, France
[3] Texas A&M Univ, Dept Small Anim Clin Sci, Gastrointestinal Lab, College Stn, TX 77843 USA
关键词
biogenic amines; dysbiosis index; gastrointestinal functionality; metagenomics; NUTRIENT DIGESTIBILITY; PROTEIN-SOURCES; FIBER; KEGG;
D O I
10.3390/microorganisms11020506
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim was to evaluate the effects of yeast probiotic on diet digestibility, fermentative metabolites, and fecal microbiota of dogs submitted to dietary change. Sixteen dogs were divided into two groups of eight dogs each: control, without, and with probiotic, receiving 0.12 g/dog/day of live Saccharomyces cerevisiae yeast. The dogs were fed a lower protein and fiber diet for 21 days and then changed to a higher protein and fiber diet until day 49. Yeast supplementation did not statistically influence diet digestibility. The probiotic group had a lower fecal concentration of total biogenic amines (days 21 and 49), ammonia (day 23), and aromatic compounds and a higher fecal concentration of butyrate (p < 0.05). The probiotic group showed a lower dysbiosis index, a higher abundance (p < 0.05) of Bifidobacterium (days 35 and 49) and Turicibacter, and a lower abundance of Lactobacillus and E. coli (p < 0.05). Beta diversity demonstrated a clear differentiation in the gut microbiota between the control and probiotic groups on day 49. The control group showed upregulation in genes related to virulence factors, antibiotic resistance, and osmotic stress. The results indicated that the live yeast evaluated can have beneficial effects on intestinal functionality of dogs.
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页数:20
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