Identity and distribution of American foulbrood (Paenibacillus larvae) in South Africa

被引：2

作者：

Hristov, Yordan, V ^{[1
]}

Le Roux, Johannes J. ^{[1
,2
]}

Allsopp, Michael H. ^{[3
]}

Wossler, Theresa C. ^{[1
]}

机构：

[1] Stellenbosch Univ, Dept Bot & Zool, Matieland, Western Cape, South Africa

[2] Macquarie Univ, Dept Biol Sci, Sydney, NSW, Australia

[3] ARC Plant Protect Res Inst, Honey Bee Res Sect, Stellenbosch, South Africa

来源：

JOURNAL OF APICULTURAL RESEARCH | 2023年 / 62卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

American foulbrood; honey bees; Paenibacillus larvae; LabChip technology; rep-PCR; South Africa; PCR;

D O I：

10.1080/00218839.2021.1887635

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

American foulbrood (AFB) is a serious and near-global disease of honey bees and was recently discovered in South Africa. The causative pathogen, Paenibacillus larvae, comprises various strains. It is currently unknown which of these are present in South Africa and what their distribution is. We address this issue by genotyping P. larvae isolates using repetitive element fingerprinting (rep-PCR) markers and microfabricated chip technology. A total of 71 brood, honey and wax samples were collected opportunistically in South Africa. Twenty-three samples tested positive for P. larvae (32.4%). Diagnostic rep-PCR fingerprinting using ERIC, BOX A1R and MBO REP1 primers found all P. larvae isolates in South Africa to correspond to the ERIC I genotype, with two subtypes revealed, Ab (13 samples) and ab (10 samples). These results are in agreement with the global prevalence of the ERIC I AFB genotype among P. larvae-infected honey bee colonies.

引用

页码：303 / 310

页数：8

共 57 条

[21] ERIC-PCR Genotyping of Paenibacillus larvae in Southern Italian Honey and Brood Combs
Di Pinto, Angela
Novello, Lucia
Terio, Valentina
Tantillo, Giuseppina
[J]. CURRENT MICROBIOLOGY, 2011, 63 (05) : 416 - 419
[22] Dietemann V, 2009, APIDOLOGIE, V40, P285, DOI 10.1051/apido/2009013
[23] MEDIUM PROMOTING SPORULATION OF BACILLUS-LARVAE AND METABOLISM OF MEDIUM COMPONENTS
DINGMAN, DW
STAHLY, DP
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (04) : 860 - 869
[24] DNA RESTRICTION-ENDONUCLEASE PROFILES AND TYPING OF GEOGRAPHICALLY DIVERSE ISOLATES OF BACILLUS-LARVAE
DJORDJEVIC, S
HOSHON, M
HORNITZKY, M
[J]. JOURNAL OF APICULTURAL RESEARCH, 1994, 33 (02) : 95 - 103
[25] Dobbelaere W, 2001, APIDOLOGIE, V32, P363, DOI 10.1051/apido:2001136
[26] The worldwide health status of honey bees
Ellis, JD
Munn, PA
[J]. BEE WORLD, 2005, 86 (04) : 88 - 101
[27] Fries I, 2001, APIDOLOGIE, V32, P199, DOI 10.1051/apido:2001122
[28] American foulbrood and African honey bees (Hymenoptera: Apidae)
Fries, I
Raina, S
[J]. JOURNAL OF ECONOMIC ENTOMOLOGY, 2003, 96 (06) : 1641 - 1646
[29] Reclassification of Paenibacillus larvae subsp pulvifaciens and Paenibacillus larvae subsp larvae as Paenibacillus larvae without subspecies differentiation
Genersch, E
Forsgren, E
Pentikäinen, J
Ashiralieva, A
Rauch, S
Kilwinski, J
Fries, I
[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 : 501 - 511
[30] Strain- and genotype-specific differences in virulence of Paenibacillus larvae subsp larvae, a bacterial pathogen causing American foulbrood disease in honeybees
Genersch, E
Ashiralieva, A
Fries, I
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) : 7551 - 7555

← 1 2 3 4 5 6 →