Study of adaptive window space direction contrast method in transmission speckle contrast imaging

Laser speckle contrast imaging (LSCI) is a non-scanning full-field hemodynamic imaging technology, which has the advantages of real-time, non-invasive, and high spatiotemporal resolution. It has become a widely used optical imaging technology for vascular visualization and dynamic blood flow monitoring. The Reflect-LSCI (R-LSCI) system is mostly used for the imaging of superficial blood vessels, exhibiting poor image quality when it comes to deep vascular visualization. Some studies have shown that Transmissive-LSCI (T-LSCI) has advantages in deep tissue imaging. At the same time, adaptive window space direction (awsdK) method has better imaging quality for deep blood vessels in R-LSCI. In this study, we used several LSCI methods processed in the spatial domain to compare the speckle images acquired by the R-LSCI system and the T-LSCI system. The results of comparative experiments show that in the T-LSCI, the awsdK also has the ability to improve the visualization of deep blood vessels without changing the relative velocity information. At the same time, the reflection speckle images and transmission speckle images were compared. The results showed that the T-LSCI was better than the R-LSCI in deep tissue imaging for a certain thickness of tissue.