Enantioseparation of two antifungal azole drugs by analytical countercurrent chromatography using sulfobutyl ether-,B-cyclodextrin as chiral selector

This study reports a successful enantioseparation of two antifungal drugs, Ketoconazole and Voriconazole, using countercurrent chromatography (CCC) with synthesized sulfobutyl ether- ,B-cyclodextrin (SBE- ,B-CD) as chiral selector. Two biphasic solvent systems composed of dichloromethane: 0.1 mol L -1 of phosphate buffer solution (pH 3.0) (1:1, v/v) and n- hexane: ethyl acetate: 0.1 mol L -1 phosphate buffer solution (pH 3.0) (1.5:0.5:2, v/v/v) were selected. Influence factors were investigated, including degree of substitution of SBE- ,B-CD, concentration of SBE- ,B-CD, equilibrium temperature, and pH of aqueous phase. Under optimized separation conditions, a large enantioseparation factor of a & GE; 3.26 and a high peak resolution R s = 1.82, was achieved for enantioseparation of Voriconazole by countercurrent chromatography, and purity of two azole stereoisomers collected from CCC separation reached 98.5%, as determined by HPLC. Molecular docking was employed to investigate the formation of inclusion complex.& COPY; 2023 Elsevier B.V. All rights reserved.