Polydimethylsiloxane assisted surface-enhanced Raman spectroscopy for Staphylococcus aureus detection based on aptamer recognition

被引：8
作者：
Zhu, Afang [1 ]
Ali, Shujat [3 ]
Wang, Zhen [1 ]
Jiao, Tianhui [2 ]
Ouyang, Qin [1 ]
Chen, Quansheng [1 ,2 ]
机构：
[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[2] Jimei Univ, Coll Food & Biol Engn, Xiamen 361021, Peoples R China
[3] Wenzhou Univ, Coll Elect & Elect Engn, Wenzhou 325035, Peoples R China
来源：
MICROCHEMICAL JOURNAL | 2023年 / 193卷
基金：
中国国家自然科学基金;
关键词：
SERS; Au@Ag NPs; Aptamer; S; aureus; Detection; SERS TAGS; BACTERIA;
D O I：
10.1016/j.microc.2023.109172
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A highly structured SERS biosensor was proposed for Staphylococcus aureus (S. aureus, ATCC 29213) detection. Polydimethylsiloxane (PDMS) film was chemically modified and gold and silver core-shell nanoparticles (Au@Ag NPs) were self-assembled on it. Sulfhydryl-modified aptamer of S. aureus was immobilized on the SERS substrate to form a capture substrate and the aptamer modified with 6-carboxy-x-rhodamine (ROX) was used as a signal molecular probe. The aptamer and target constructed a sandwich structure "capturing substrate-targetsignal probe". Concurrently, the bacterial surface was covered with Au@Ag NPs which resulted in more "hot spots" and improved the detection sensitivity. The SERS intensity was plotted against the logarithmic concentration of S. aureus (3.6 x 10 to 3.6 x 10(8) cfu/mL) and achieved an ultra-low detection response of 16 cfu/mL. S. aureus was detected in tap water, milk and fish samples with satisfactory recoveries (93.25%similar to 106.42%) and validation results (p > 0.05). Significantly, the method offered a speedy and accurate S. aureus examining in food/environmental settings.
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页数：8
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