Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells

被引：6

作者：

Cahyadi, Danang Dwi ^{[1
,2
]}

Warita, Tomoko ^{[3
,7
]}

Irie, Nanami ^{[4
]}

Mizoguchi, Kana ^{[4
]}

Tashiro, Jiro ^{[1
]}

Hosaka, Yoshinao Z. ^{[5
]}

Warita, Katsuhiko ^{[1
,6
]}

机构：

[1] Tottori Univ, Joint Grad Sch Vet Sci, Tottori, Tottori, Japan

[2] IPB Univ, Sch Vet Med & Biomed Sci, Div Anat Histol & Embryol, Bogor, West Java, Indonesia

[3] Kwansei Gakuin Univ, Sch Biol & Environm Sci, Dept Biomed Sci, Sanda, Hyogo, Japan

[4] Kwansei Gakuin Univ, Grad Sch Sci & Technol, Sanda, Hyogo, Japan

[5] Kyushu Univ, Fac Agr, Dept Bioresource Sci, Fukuoka, Fukuoka, Japan

[6] Tottori Univ, Fac Agr, Joint Dept Vet Med, Tottori, Tottori, Japan

[7] Kwansei Gakuin Univ, Sch Biol & Environm Sci, Dept Biomed Sci, 1 Gakuen Uegahara, Sanda, Hyogo 6691330, Japan

来源：

ADIPOCYTE | 2023年 / 12卷 / 01期

基金：

日本学术振兴会;

关键词：

adipocytes; differentiation; gene expression; normalization; reference gene; MESSENGER-RNA; QUANTITATIVE PCR; RT-PCR; ADIPOCYTES; INSULIN; MOUSE; OBESITY; LINE; NORMALIZATION; VALIDATION;

D O I：

10.1080/21623945.2023.2235081

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Normalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and differentiated (DI) 3T3-L1 cells on days 5 and 10. We used geNorm, NormFinder, BestKeeper, RefFinder, and the increment Ct method to evaluate expression stability. The findings revealed that (1) the expression levels of the reference genes changed over time, even in non-differentiating cells, and (2) peptidylprolyl isomerase A (Ppia) and TATA box-binding protein (Tbp) were stable reference genes for 10 days in both undifferentiated and differentiated 3T3-L1 cells. Notably, the expression of known reference genes in non-differentiating cells was altered throughout the experiment.

引用

页数：13

共 63 条

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