Characterizing the biology of primary brain tumors and their microenvironment via single-cell profiling methods

被引：26

作者：

Gonzalez Castro, L. Nicolas ^{[1
,2
]}

Liu, Ilon ^{[3
]}

Filbin, Mariella ^{[3
]}

机构：

[1] Brigham & Womens Hosp, Dept Neurol, Boston, MA USA

[2] Dana Farber Canc Inst, Ctr Neurooncol, Boston, MA 02215 USA

[3] Dana Farber Boston Childrens & Blood Disorders Ct, Pediat Neuro Oncol Program, Boston, MA USA

来源：

NEURO-ONCOLOGY | 2023年 / 25卷 / 02期

关键词：

gliomas; medulloblastoma; single-cell methods; singlecell RNA-seq; single-cell multi-omics; CENTRAL-NERVOUS-SYSTEM; EMBRYONIC STEM-CELLS; RNA-SEQ; METHYLOME LANDSCAPES; ENHANCER ACTIVITY; DRIVER MUTATIONS; HISTONE H3.3; MODEL; GLIOBLASTOMA; CHROMATIN;

D O I：

10.1093/neuonc/noac211

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Genomic and transcriptional heterogeneity is prevalent among the most common and aggressive primary brain tumors in children and adults. Over the past 20 years, advances in bioengineering, biochemistry and bioinformatics have enabled the development of an array of techniques to study tumor biology at single-cell resolution. The application of these techniques to study primary brain tumors has helped advance our understanding of their intra-tumoral heterogeneity and uncover new insights regarding their co-option of developmental programs and signaling from their microenvironment to promote tumor proliferation and invasion. These insights are currently being harnessed to develop new therapeutic approaches. Here we provide an overview of current single-cell techniques and discuss relevant biology and therapeutic insights uncovered by their application to primary brain tumors in children and adults.

引用

页码：234 / 247

页数：14

共 136 条

[1] A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response [J].

Adamson, Britt ;

Norman, Thomas M. ;

Jost, Marco ;

Cho, Min Y. ;

Nunez, James K. ;

Chen, Yuwen ;

Villalta, Jacqueline E. ;

Gilbert, Luke A. ;

Horlbeck, Max A. ;

Hein, Marco Y. ;

Pak, Ryan A. ;

Gray, Andrew N. ;

Gross, Carol A. ;

Dixit, Atray ;

Parnas, Oren ;

Regev, Aviv ;

Weissman, Jonathan S. .

CELL, 2016, 167 (07) :1867-+

[2] Multiplexed ion beam imaging of human breast tumors [J].

Angelo, Michael ;

Bendall, Sean C. ;

Finck, Rachel ;

Hale, Matthew B. ;

Hitzman, Chuck ;

Borowsky, Alexander D. ;

Levenson, Richard M. ;

Lowe, John B. ;

Liu, Scot D. ;

Zhao, Shuchun ;

Natkunam, Yasodha ;

Nolan, Garry P. .

NATURE MEDICINE, 2014, 20 (04) :436-+

[3] Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity [J].

Angermueller, Christof ;

Clark, Stephen J. ;

Lee, Heather J. ;

Macaulay, Iain C. ;

Teng, Mabel J. ;

Hu, Tim Xiaoming ;

Krueger, Felix ;

Smallwood, Sebastien A. ;

Ponting, Chris P. ;

Voet, Thierry ;

Kelsey, Gavin ;

Stegle, Oliver ;

Reik, Wolf .

NATURE METHODS, 2016, 13 (03) :229-+

[4] Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization [J].

Antunes, Ana Rita Pombo ;

Scheyltjens, Isabelle ;

Lodi, Francesca ;

Messiaen, Julie ;

Antoranz, Asier ;

Duerinck, Johnny ;

Kancheva, Daliya ;

Martens, Liesbet ;

De Vlaminck, Karen ;

Van Hove, Hannah ;

Hansen, Signe Schmidt Kjolner ;

Bosisio, Francesca Maria ;

Van der Borght, Koen ;

De Vleeschouwer, Steven ;

Sciot, Raf ;

Bouwens, Luc ;

Verfaillie, Michiel ;

Vandamme, Niels ;

Vandenbroucke, Roosmarijn E. ;

De Wever, Olivier ;

Saeys, Yvan ;

Guilliams, Martin ;

Gysemans, Conny ;

Neyns, Bart ;

De Smet, Frederik ;

Lambrechts, Diether ;

Van Ginderachter, Jo A. ;

Movahedi, Kiavash .

NATURE NEUROSCIENCE, 2021, 24 (04) :595-610

[5] ZFTA-RELA Dictates Oncogenic Transcriptional Programs to Drive Aggressive Supratentorial Ependymoma [J].

Arabzade, Amir ;

Zhao, Yanhua ;

Varadharajan, Srinidhi ;

Chen, Hsiao-Chi ;

Jessa, Selin ;

Rivas, Bryan ;

Stuckert, Austin J. ;

Solis, Minerva ;

Kardian, Alisha ;

Tlais, Dana ;

Golbourn, Brian J. ;

Stanton, Ann-Catherine J. ;

Chan, Yuen San ;

Olson, Calla ;

Karlin, Kristen L. ;

Kong, Kathleen ;

Kupp, Robert ;

Hu, Baoli ;

Injac, Sarah G. ;

Ngo, Madeline ;

Wang, Peter R. ;

De Leon, Luz A. ;

Sahm, Felix ;

Kawauchi, Daisuke ;

Pfister, Stefan M. ;

Lin, Charles Y. ;

Hodges, H. Courtney ;

Singh, Irtisha ;

Westbrook, Thomas F. ;

Chintagumpala, Murali M. ;

Blaney, Susan M. ;

Parsons, Donald W. ;

Pajtler, Kristian W. ;

Agnihotri, Sameer ;

Gilbertson, Richard J. ;

Yi, Joanna ;

Jabado, Nada ;

Kleinman, Claudia L. ;

Bertrand, Kelsey C. ;

Deneen, Benjamin ;

Mack, Stephen C. .

CANCER DISCOVERY, 2021, 11 (09) :2200-2215

[6] ATRX regulates glial identity and the tumor microenvironment in IDH-mutant glioma [J].

Babikir, Husam ;

Wang, Lin ;

Shamardani, Karin ;

Catalan, Francisca ;

Sudhir, Sweta ;

Aghi, Manish K. ;

Raleigh, David R. ;

Phillips, Joanna J. ;

Diaz, Aaron A. .

GENOME BIOLOGY, 2021, 22 (01)

[7] Experimental design for single-cell RNA sequencing [J].

Baran-Gale, Jeanette ;

Chandra, Tamir ;

Kirschner, Kristina .

BRIEFINGS IN FUNCTIONAL GENOMICS, 2018, 17 (04) :233-239

[8] Reduced H3K27me3 and DNA Hypomethylation Are Major Drivers of Gene Expression in K27M Mutant Pediatric High-Grade Gliomas [J].

Bender, Sebastian ;

Tang, Yujie ;

Lindroth, Anders M. ;

Hovestadt, Volker ;

Jones, David T. W. ;

Kool, Marcel ;

Zapatka, Marc ;

Northcott, Paul A. ;

Sturm, Dominik ;

Wang, Wei ;

Radlwimmer, Bernhard ;

Hojfeldt, Jonas W. ;

Truffaux, Nathalene ;

Castel, David ;

Schubert, Simone ;

Ryzhova, Marina ;

Seker-Cin, Huriye ;

Gronych, Jan ;

Johann, Pascal David ;

Stark, Sebastian ;

Meyer, Jochen ;

Milde, Till ;

Schuhmann, Martin ;

Ebinger, Martin ;

Monoranu, Camelia-Maria ;

Ponnuswami, Anitha ;

Chen, Spenser ;

Jones, Chris ;

Witt, Olaf ;

Collins, V. Peter ;

von Deimling, Andreas ;

Jabado, Nada ;

Puget, Stephanie ;

Grill, Jacques ;

Helin, Kristian ;

Korshunov, Andrey ;

Lichter, Peter ;

Monje, Michelle ;

Plass, Christoph ;

Cho, Yoon-Jae ;

Pfister, Stefan M. .

CANCER CELL, 2013, 24 (05) :660-672

[9] Histone H3.3 Mutations Drive Pediatric Glioblastoma through Upregulation of MYCN [J].

Bjerke, Lynn ;

Mackay, Alan ;

Nandhabalan, Meera ;

Burford, Anna ;

Jury, Alexa ;

Popov, Sergey ;

Bax, Dorine A. ;

Carvalho, Diana ;

Taylor, Kathryn R. ;

Vinci, Maria ;

Bajrami, Ilirjana ;

McGonnell, Imelda M. ;

Lord, Christopher J. ;

Reis, Rui M. ;

Hargrave, Darren ;

Ashworth, Alan ;

Workman, Paul ;

Jones, Chris .

CANCER DISCOVERY, 2013, 3 (05) :512-519

[10] Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants [J].

Bressan, Raul Bardini ;

Southgate, Benjamin ;

Ferguson, Kirsty M. ;

Blin, Carla ;

Grant, Vivien ;

Alfazema, Neza ;

Wills, Jimi C. ;

Marques-Torrejon, Maria Angeles ;

Morrison, Gillian M. ;

Ashmore, James ;

Robertson, Faye ;

Williams, Charles A. C. ;

Bradley, Leanne ;

von Kriegsheim, Alex ;

Anderson, Richard A. ;

Tomlinson, Simon R. ;

Pollard, Steven M. .

CELL STEM CELL, 2021, 28 (05) :877-+

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