Systematic study on lysozyme-hyaluronan complexes: Multi-spectroscopic characterization and molecular dynamics simulation

This study systematically investigated the complexation mechanism of lysozyme (LYS) and hyaluronan (HA) as well as their complex-formation process using multi-spectroscopy combined with molecular dynamics simulation. Overall, the results demonstrated that electrostatic interaction provides the primary self-assembly driving forces for LYS-HA complex formation. Circular dichroism spectroscopy revealed that the LYS-HA complexes formation primarily alters the alpha-helix and beta-sheet structures of LYS. Fluorescence spectroscopy yielded an entropy of 0.12 kJ/mol center dot K and enthalpy of -44.46 kJ/mol for LYS-HA complexes. Molecular dynamics simulation indicated that the amino acid residues of ARG114 in LYS and 4ZB4 in HA contributed most significantly. HT-29 and HCT-116 cell experiments demonstrated that LYS-HA complexes possess excellent biocompatibility. Furthermore, LYS-HA complexes were found to be potentially useful the efficient encapsulation of several insoluble drugs and bioactives. These findings provide new insight into the binding mechanism between LYS and HA, and prove indispensable to promoting the potential application of LYS-HA complexes as bioactive compound delivery systems, emulsion stabilizers, or foaming agents in the food industry.