Transcriptome-Wide Identification of the GRAS Transcription Factor Family in Pinus massoniana and Its Role in Regulating Development and Stress Response

被引:2
作者
Yang, Ye [1 ]
Agassin, Romaric Hippolyte [1 ]
Ji, Kongshu [1 ]
机构
[1] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China
关键词
Pinus massoniana; GRAS; abiotic stresses; hormone treatments; development; expression; GENE FAMILY; ARABIDOPSIS; EXPRESSION; PROTEINS; TOLERANCE; SIGNAL; ORGANIZATION; PHYTOCHROME; COMPLEX; CLONING;
D O I
10.3390/ijms241310690
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pinus massoniana is a species used in afforestation and has high economic, ecological, and therapeutic significance. P. massoniana experiences a variety of biotic and abiotic stresses, and thus presents a suitable model for studying how woody plants respond to such stress. Numerous families of transcription factors are involved in the research of stress resistance, with the GRAS family playing a significant role in plant development and stress response. Though GRASs have been well explored in various plant species, much research remains to be undertaken on the GRAS family in P. massoniana. In this study, 21 PmGRASs were identified in the P. massoniana transcriptome. P. massoniana and Arabidopsis thaliana phylogenetic analyses revealed that the PmGRAS family can be separated into nine subfamilies. The results of qRT-PCR and transcriptome analyses under various stress and hormone treatments reveal that PmGRASs, particularly PmGRAS9, PmGRAS10 and PmGRAS17, may be crucial for stress resistance. The majority of PmGRASs were significantly expressed in needles and may function at multiple locales and developmental stages, according to tissue-specific expression analyses. Furthermore, the DELLA subfamily members PmGRAS9 and PmGRAS17 were nuclear localization proteins, while PmGRAS9 demonstrated transcriptional activation activity in yeast. The results of this study will help explore the relevant factors regulating the development of P. massoniana, improve stress resistance and lay the foundation for further identification of the biological functions of PmGRASs.
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页数:20
相关论文
共 69 条
[1]   The GRAS gene family in pine: transcript expression patterns associated with the maturation-related decline of competence to form adventitious roots [J].
Abarca, Dolores ;
Pizarro, Alberto ;
Hernandez, Inmaculada ;
Sanchez, Conchi ;
Solana, Silvia P. ;
del Amo, Alicia ;
Carneros, Elena ;
Diaz-Sala, Carmen .
BMC PLANT BIOLOGY, 2014, 14
[2]   Gene Regulation via the Combination of Transcription Factors in the INDETERMINATE DOMAIN and GRAS Families [J].
Aoyanagi, Takuya ;
Ikeya, Shun ;
Kobayashi, Atsushi ;
Kozaki, Akiko .
GENES, 2020, 11 (06) :1-18
[3]   Regulation of DELLA Proteins by Post-translational Modifications [J].
Blanco-Tourinan, Noel ;
Serrano-Mislata, Antonio ;
Alabadi, David .
PLANT AND CELL PHYSIOLOGY, 2020, 61 (11) :1891-1901
[4]  
Bolle C, 2000, GENE DEV, V14, P1269
[5]   The role of GRAS proteins in plant signal transduction and development [J].
Bolle, C .
PLANTA, 2004, 218 (05) :683-692
[6]   Elucidating the evolutionary conserved DNA-binding specificities of WRKY transcription factors by molecular dynamics and in vitro binding assays [J].
Brand, Luise H. ;
Fischer, Nina M. ;
Harter, Klaus ;
Kohlbacher, Oliver ;
Wanke, Dierk .
NUCLEIC ACIDS RESEARCH, 2013, 41 (21) :9764-9778
[7]   Evolutionary Analyses of GRAS Transcription Factors in Angiosperms [J].
Cenci, Alberto ;
Rouard, Mathieu .
FRONTIERS IN PLANT SCIENCE, 2017, 8
[8]   TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].
Chen, Chengjie ;
Chen, Hao ;
Zhang, Yi ;
Thomas, Hannah R. ;
Frank, Margaret H. ;
He, Yehua ;
Xia, Rui .
MOLECULAR PLANT, 2020, 13 (08) :1194-1202
[9]   Identification and Expression Analysis of GRAS Transcription Factors to Elucidate Candidate Genes Related to Stolons, Fruit Ripening and Abiotic Stresses in Woodland Strawberry (Fragaria vesca) [J].
Chen, Hong ;
Li, Huihui ;
Lu, Xiaoqing ;
Chen, Longzheng ;
Liu, Jing ;
Wu, Han .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (18)
[10]   Bundling up: VaPAT1 forms a complex with VaIDD3 to activate cold tolerance in Amur grape calli [J].
Courbier, Sarah .
PLANT PHYSIOLOGY, 2021, 186 (03) :1373-1374