Exosomes derived from berberine-treated bone marrow mesenchymal stem cells ameliorate inflammatory arthritis in rats with collagen-induced rheumatoid arthritis

被引：6
作者：
Sun, Fei [1 ]
Ma, Yuxia [2 ]
Li, Faxin [3 ]
Li, Congcong [4 ]
Yang, Zhaowen [2 ]
机构：
[1] Shandong First Med Univ, Dept Integrated Tradit Chinese & Western Med, Cent Hosp, Jinan, Peoples R China
[2] Shandong First Med Univ, Cent Hosp, Dept Rheumatol & Immunol, Jinan, Peoples R China
[3] Shandong Second Prov Gen Hosp, Dept Rheumatol, Jinan, Peoples R China
[4] Shandong First Med Univ, Dept Gen Practice, Cent Hosp, Jinan, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2023年 / 34卷 / 01期
关键词：
Berberine; bone marrow mesenchymal stem cells; exosomes; rheumatoid arthritis; synovial inflammation; REGULATORY T-CELLS; FIBROBLAST-LIKE; TNF-ALPHA; JOINT INFLAMMATION; IN-VITRO; DESTRUCTION; MACROPHAGES; RESPONSES; IL-17; TH17;
D O I：
10.1080/09540105.2023.2220566
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
To evaluate the effect of exosomes secreted by berberine-treated bone marrow-derived mesenchymal stem cells (BMSCs) on synovial inflammation in collagen-induced arthritis (CIA) model. Exosomes were isolated from berberine-treated BMSCs (Ber-BMSC-EXs) and untreated BMSCs (BMSC-EXs). CIA rats were developed and treated with Ber-BMSC-EXs or BMSC-EXs. Clinical arthritis index was measured, activation of fibroblast-like synoviocytes (FLSs) were evaluated, and response and frequency of T helper 17 (Th17) and regulatory T (Treg) cells as well as cytokine levels in synovial fluid were determined. Ber-BMSC-EXs (with a superior effect than BMSC-EXs) significantly alleviated RA clinical score and paw inflammation, balanced Th17 and Treg cells' responses and population, modulated levels of pro-inflammatory and anti-inflammatory cytokines in joint synovial fluid, and inhibited FLS activation in CIA rats. Ber-BMSC-EXs (with a significantly greater effect than BMSC-EXs) could significantly ameliorate synovial joint inflammation and clinical symptoms of arthritis in CIA rats, showing that berberine can reinforce anti-arthritic effects of BMSC-EXs.
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页数：22
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