Construction of a cross-species cell landscape at single-cell level

被引：47

作者：

Wang, Renying ^{[1
,2
]}

Zhang, Peijing ^{[1
,2
,3
]}

Wang, Jingjing ^{[1
,2
,3
]}

Ma, Lifeng ^{[1
,2
]}

Weigao, E. ^{[1
,2
]}

Suo, Shengbao ^{[4
]}

Jiang, Mengmeng ^{[1
,2
,3
]}

Li, Jiaqi ^{[1
,2
]}

Chen, Haide ^{[1
,2
,3
]}

Sun, Huiyu ^{[1
,2
]}

Fei, Lijiang ^{[1
,2
]}

Zhou, Ziming ^{[1
,2
]}

Zhou, Yincong ^{[5
]}

Chen, Yao ^{[6
]}

Zhang, Weiqi ^{[7
]}

Wang, Xinru ^{[1
,2
]}

Mei, Yuqing ^{[1
,2
]}

Sun, Zhongyi ^{[1
,2
]}

Yu, Chengxuan ^{[1
,2
]}

Shao, Jikai ^{[1
,2
]}

Fu, Yuting ^{[1
,2
]}

Xiao, Yanyu ^{[1
,2
]}

Ye, Fang ^{[1
,2
]}

Fang, Xing ^{[1
,2
]}

Wu, Hanyu ^{[1
,2
]}

Guo, Qile ^{[8
]}

Fang, Xiunan ^{[1
,2
]}

Li, Xia ^{[1
,2
]}

Gao, Xianzhi ^{[9
,10
]}

Wang, Dan ^{[11
,12
]}

Xu, Peng-Fei ^{[11
,12
]}

Zeng, Rui ^{[13
]}

Xu, Gang ^{[13
]}

Zhu, Lijun ^{[14
]}

Wang, Lie ^{[9
,10
]}

Qu, Jing ^{[15
]}

Zhang, Dan ^{[6
]}

Ouyang, Hongwei ^{[8
,16
]}

Huang, He ^{[1
,2
]}

Chen, Ming ^{[5
]}

Ng, Shyh-Chang ^{[15
]}

Liu, Guang-Hui ^{[15
]}

Yuan, Guo-Cheng ^{[17
]}

Guo, Guoji ^{[1
,2
,3
,8
,16
]}

Han, Xiaoping ^{[1
,2
,16
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Ctr Stem Cell & Regenerat Med, Hangzhou 310000, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Hangzhou 310000, Zhejiang, Peoples R China

[3] Zhejiang Univ, Liangzhu Lab, Med Ctr, Hangzhou 311121, Peoples R China

[4] Guangzhou Lab, Guangzhou 510005, Peoples R China

[5] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China

[6] Zhejiang Univ, Womens Hosp, Dept Reprod Endocrinol, Key Lab Reprod Genet,Minist Educ,Sch Med, Hangzhou 310006, Zhejiang, Peoples R China

[7] Chinese Acad Sci, China Natl Ctr Bioinformat, Beijing Inst Genom, CAS Key Lab Genom & Precis Med, Beijing 100101, Peoples R China

[8] Zhejiang Univ, Sch Med, Zhejiang Univ Univ Edinburgh Inst, Hangzhou 314400, Peoples R China

[9] Zhejiang Univ, Affiliated Hosp 1, Inst Immunol, Sch Med, Hangzhou 310058, Peoples R China

[10] Zhejiang Univ, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Sch Med, Hangzhou 310058, Peoples R China

[11] Zhejiang Univ, Womens Hosp, Sch Med, Hangzhou 310058, Peoples R China

[12] Zhejiang Univ, Inst Genet, Sch Med, Hangzhou 310058, Peoples R China

[13] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Div Nephrol, Wuhan 430030, Peoples R China

[14] Zhejiang Univ, Affiliated Hosp 1, Zhejiang Prov Key Lab Diag & Treatment Aging & Ph, Sch Med, Hangzhou 310058, Peoples R China

[15] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China

[16] Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Zhejiang Prov Key Lab Tissue Engn & Regenerat Med, Hangzhou 310058, Zhejiang, Peoples R China

[17] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, Dept Genet & Genom Sci, New York, NY 10029 USA

来源：

NUCLEIC ACIDS RESEARCH | 2023年 / 51卷 / 02期

基金：

中国国家自然科学基金;

关键词：

MESSENGER-RNA-SEQ; EXPRESSION; DIVERSITY; MITOCHONDRIA; MUSCLE;

D O I：

10.1093/nar/gkac633

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Individual cells are basic units of life. Despite extensive efforts to characterize the cellular heterogeneity of different organisms, cross-species comparisons of landscape dynamics have not been achieved. Here, we applied single-cell RNA sequencing (scRNA-seq) to map organism-level cell landscapes at multiple life stages for mice, zebrafish and Drosophila. By integrating the comprehensive dataset of > 2.6 million single cells, we constructed a cross-species cell landscape and identified signatures and common pathways that changed throughout the life span. We identified structural inflammation and mitochondrial dysfunction as the most common hallmarks of organism aging, and found that pharmacological activation of mitochondrial metabolism alleviated aging phenotypes in mice. The cross-species cell landscape with other published datasets were stored in an integrated online portal-Cell Landscape. Our work provides a valuable resource for studying lineage development, maturation and aging. [GRAPHICS] .

引用

页码：501 / 516

页数：16

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