Long non-coding RNA SNHG5 promotes osteogenic differentiation of human periodontal ligament stem cells via mediating miR-23b-3p/Runx2 axis

被引:6
|
作者
Sun, Xuefei [1 ,2 ]
Li, Zhidan [1 ,2 ]
Dong, Shaojie [1 ,2 ]
Dong, Qianqian [1 ,2 ]
机构
[1] Xi An Jiao Tong Univ, Coll Stomatol, Key Lab Shaanxi Prov Craniofacial Precis Med Res, Xian, Peoples R China
[2] Xi An Jiao Tong Univ, Coll Stomatol, Clin Res Ctr Shaanxi Prov Dent & Maxillofacial Dis, Dept Endodont, Xian, Peoples R China
来源
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | 2023年 / 20卷 / 07期
基金
中国国家自然科学基金;
关键词
SNHG5; osteogenic differentiation; hPDLSCs (human periodontal ligament stem cells); miR-23b-3p;
D O I
10.7150/ijms.82454
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The treatment of bone loss due to periodontitis has posed a great challenge for physicians for decades. Therefore, it is of extraordinary significance to identify an effective regeneration scheme for alveolar bone. This study aimed to investigate long non-coding RNA (lncRNA) small nucleolar RNA host gene 5 (SNHG5) whether sponges microRNA-23b-3p (miR-23b-3p) to achieve the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). Results revealed that the expression of SNHG5 was upregulated whereas that of miR-23b-3p was downregulated in osteogenic hPDLSCs. Alizarin red staining assays and qRT-PCR demonstrated that SNHG5 silencing or miR-23b-3p overexpression inhibits hPDLSCs osteogenic differentiation and vice versa. In addition, miR-23b-3p partially abolished the promotive effect of SNHG5 on osteogenic differentiation of hPDLSCs. Dual luciferase report and RNA pulldown assay verified that miR-23b-3p is a regulatory target of SNHG5 and that Runx2 is a gene target of miR-23b-3p. In brief, the results demonstrate that SNHG5 promotes the osteogenic differentiation of hPDLSCs by regulating the miR-23b-3p/Runx2 axis. Our study provides novel mechanistic insights into the critical role of lncRNA SNHG5 as a miR-23b-3p sponge to regulate Runx2 expression in hPDLSCs and may serve as a potential therapeutics target for periodontitis.
引用
收藏
页码:958 / 968
页数:11
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