Enzymatic biodegradation, kinetic study, and detoxification of Reactive Red-195 by Halomonas meridiana isolated from Marine Sediments of Andaman Sea, India

Azo dyes are a significant class of hazardous chemicals that are extensively utilised in diverse industries. Industries that manufacture and consume reactive azo dyes generate hyper-saline wastewater. The ability of halotolerant bacteria to thrive under extreme environmental conditions thus makes them a potential candidate for reactive azo dye degradation. An efficient halotolerant bacterium (isolate SAIBP-6) with the capability to degrade 87.15% of azo dye Reactive Red 195 (RR-195) was isolated from sea sediment and identified as Halomonas meridiana SAIBP-6. Strain SAIBP-6 maintained potential decolourisation under a wide range of environmental conditions viz. 35-45 degrees C temperature, 50-450 mg/L RR-195, pH 7-9, and 50-150 g/L NaCl. However, maximum decolourisation occurred at 40 degrees C, 200 mg/L RR-195 dye, pH 9, and 50 g/L NaCl, under static conditions. Tyrosinase and azoreductase were responsible for dye degradation. The reaction catalysed by these enzymes followed zero-order kinetics. The maximum velocity (V-max) of the enzymatic reaction was 4.221 mg/(L.h) and the Michaelis constant (K-m) was 517.982 mg/L. Strain SAIBP-6 also efficiently decolourised Reactive Black-5 and Reactive Yellow-160 dye. The biodegradation process was further studied with the help of UV-Vis spectral scan, ultra-high performance liquid chromatography (UPLC), fourier-transform infra-red spectroscopy (FT-IR), and proton nuclear magnetic resonance (H-1 NMR) analysis. Finally, cytogenotoxicity assay conducted with the meristematic root tip cells of Allium cepa and phytotoxicity assay conducted with the seeds of Vigna mungo led to the inference that strain SAIBP-6 significantly reduced the toxicity of RR-195 after biodegradation. [GRAPHICS] .