SlGH3.15, a member of the GH3 gene family, regulates lateral root development and gravitropism response by modulating auxin homeostasis in tomato

被引：11

作者：

Ai, Guo ^{[1
]}

Huang, Rong ^{[1
]}

Zhang, Dedi ^{[1
]}

Li, Miao ^{[1
]}

Li, Guobin ^{[1
]}

Li, Wangfang ^{[1
]}

Ahiakpa, John K. ^{[1
]}

Wang, Yikui ^{[2
]}

Hong, Zonglie ^{[3
]}

Zhang, Junhong ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Hubei, Peoples R China

[2] Guangxi Acad Agr Sci, Vegetable Res Inst, Nanning 530007, Guangxi, Peoples R China

[3] Univ Idaho, Dept Plant Sci, Moscow, ID 83844 USA

来源：

PLANT SCIENCE | 2023年 / 330卷

关键词：

Auxin; Hormonal homeostasis; Solanum lycopersicum; Root formation; GH3; gene; SALICYLIC-ACID; GH3; FAMILY; ARABIDOPSIS; BIOSYNTHESIS; EXPRESSION; FRUIT; TRYPTOPHAN; PATHWAY; CONJUGATION; TRANSPORT;

D O I：

10.1016/j.plantsci.2023.111638

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple Gretchen Hagen 3 (GH3) genes have been implicated in a range of processes in plant growth and development through their roles in maintaining hormonal homeostasis. However, there has only been limited study on the functions of GH3 genes in tomato (Solanum lycopersicum). In this work, we investigated the important function of SlGH3.15, a member of the GH3 gene family in tomato. Overexpression of SlGH3.15led to severe dwarfism in both the above-and below-ground sections of the plant, accompanied by a substantial decrease in free IAA content and reduction in the expression of SlGH3.9, a paralog of SlGH3.15. Exogenous supply of IAA negatively affected the elongation of the primary root and partially restored the gravitropism defects in SlGH3.15-overexpression lines. While no phenotypic change was observed in the SlGH3.15 RNAi lines, double knockout lines of SlGH3.15 and SlGH3.9 were less sensitive to treatments with the auxin polar transport inhibitor. Overall, these findings revealed important roles of SlGH3.15 in IAA homeostasis and as a negative regulator of free IAA accumulation and lateral root formation in tomato.

引用

页数：10