Sensitive electrochemical biosensor for bisphenol A based on laccase immobilized on polypyrrole-3-carboxylic/Sb2O5/reduced graphene oxide hybrid nanomaterial

An electrochemical biosensor for the bisphenol A determination was constructed onto a glassy carbon electrode modified with a hybrid nanostructure based on polypyrrole-3-carboxylic acid/Sb2O5/reduced graphene oxide and laccase enzyme. The hybrid nanostructure was characterized by X-ray photoelectron spectroscopy (XPS), HR-TEM, SEM microscopy, and electrochemical techniques. The biosensor displayed excellent response for bisphenol A determinations by differential pulse voltammetry, showing a theoretical detection limit of 9.9 nmol L-1 in the linear range of 0.1-1.0 mu mol L-1. The biosensor demonstrated excellent selectivity in the determinations of bisphenol A in tap water without significant interference from other species, such as 17 beta-estradiol, Estriol, Progesterone, Catechol, Hydroquinone, Ascorbic acid, and Dopamine. The biosensor also has presented an excellent performance in bisphenol A determinations in water, with recovery tax ranging from 99.6 to 101%. Therefore, it can be used satisfactorily to detect bisphenol A in real samples.