Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

被引：61

作者：

Alvarez, Zaida ^{[1
,7
,9
]}

Ortega, J. Alberto ^{[2
,10
]}

Sato, Kohei ^{[1
,3
]}

Sasselli, Ivan R. ^{[1
,3
,11
]}

Kolberg-Edelbrock, Alexandra N. ^{[1
,4
]}

Qiu, Ruomeng ^{[1
,3
]}

Marshall, Kelly A. ^{[2
]}

Nguyen, Thao Phuong ^{[2
,4
]}

Smith, Cara S. ^{[1
,4
]}

Quinlan, Katharina A. ^{[5
]}

Papakis, Vasileios ^{[2
]}

Syrgiannis, Zois ^{[1
,3
]}

Sather, Nicholas A. ^{[1
]}

Musumeci, Chiara ^{[6
]}

Engel, Elisabeth ^{[9
]}

Stupp, Samuel I. ^{[1
,3
,4
,7
]}

Kiskinis, Evangelos ^{[1
,2
,8
]}

机构：

[1] Northwestern Univ, Simpson Querrey Inst BioNanotechnol, Chicago, IL 60611 USA

[2] Northwestern Univ, Feinberg Sch Med, Ken & Ruth Davee Dept Neurol, Chicago, IL 60611 USA

[3] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

[4] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA

[5] Univ Rhode Isl, Coll Pharm, Dept Biomed & Pharmaceut Sci, Kingston, RI 02881 USA

[6] Northwestern Univ, Dept Mat Sci & Engn, Evanston, IL 60208 USA

[7] Northwestern Univ, Dept Med, Chicago, IL 60611 USA

[8] Northwestern Univ, Feinberg Sch Med, Dept Neurosci, Chicago, IL 60611 USA

[9] Inst Bioengn Catalonia IBEC, Biomat Regenerat Therapies, Barcelona 08028, Spain

[10] Univ Barcelona, Fac Med & Hlth Sci, Dept Pathol & Expt Therapeut, LHospitalet Llobregat, Barcelona 08907, Spain

[11] Basque Res & Technol Alliance BRTA, Ctr Cooperat Res Biomat CIC biomaGUNE, Donostia San Sebastian 20014, Spain

来源：

CELL STEM CELL | 2023年 / 30卷 / 02期

基金：

美国国家卫生研究院;

关键词：

COARSE-GRAINED MODEL; SPINAL-CORD; SUPRAMOLECULAR MATERIALS; PEPTIDE AMPHIPHILES; STATISTICAL-MODEL; FORCE-FIELD; MIGRATION; LAMININ; NANOSTRUCTURES; DIFFERENTIATION;

D O I：

10.1016/j.stem.2022.12.010

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human induced pluripotent stem cell (hiPSC) technologies offer a unique resource for modeling neurological diseases. However, iPSC models are fraught with technical limitations including abnormal aggregation and inefficient maturation of differentiated neurons. These problems are in part due to the absence of synergistic cues of the native extracellular matrix (ECM). We report on the use of three artificial ECMs based on peptide amphiphile (PA) supramolecular nanofibers. All nanofibers display the laminin-derived IKVAV signal on their surface but differ in the nature of their non-bioactive domains. We find that nanofibers with greater intensity of internal supramolecular motion have enhanced bioactivity toward hiPSC-derived motor and cortical neurons. Proteomic, biochemical, and functional assays reveal that highly mobile PA scaffolds caused enhanced b1-integrin pathway activation, reduced aggregation, increased arborization, and matured electrophysiolog-ical activity of neurons. Our work highlights the importance of designing biomimetic ECMs to study the devel-opment, function, and dysfunction of human neurons.

引用

页码：219 / +

页数：35

共 127 条

[1]

Abraham Mark James, 2015, SoftwareX, V1-2, P19, DOI [10.1016/j.softx.2015.06.001, 10.1016/j.softx.2015.06.001]

[2] Functional Supramolecular Polymers [J].

Aida, T. ;

Meijer, E. W. ;

Stupp, S. I. .

SCIENCE, 2012, 335 (6070) :813-817

[3] Synthetic alternatives to Matrigel [J].

Aisenbrey, Elizabeth A. ;

Murphy, William L. .

NATURE REVIEWS MATERIALS, 2020, 5 (07) :539-551

[4] BDNF/MAPK/ERK-Induced BMP7 Expression in the Developing Cerebral Cortex Induces Premature Radial Glia Differentiation and Impairs Neuronal Migration [J].

Alberto Ortega, Juan ;

Alcantara, Soledad .

CEREBRAL CORTEX, 2010, 20 (09) :2132-2144

[5] Bioactive scaffolds with enhanced supramolecular motion promote recovery from spinal cord injury [J].

Alvarez, Z. ;

Kolberg-Edelbrock, A. N. ;

Sasselli, I. R. ;

Ortega, J. A. ;

Qiu, R. ;

Syrgiannis, Z. ;

Mirau, P. A. ;

Chen, F. ;

Chin, S. M. ;

Weigand, S. ;

Kiskinis, E. ;

Stupp, S., I .

SCIENCE, 2021, 374 (6569) :848-+

[6] Extracellular Matrix: Functions in the Nervous System [J].

Barros, Claudia S. ;

Franco, Santos J. ;

Mueller, Ulrich .

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2011, 3 (01) :1-24

[7] Extracellular matrix in the CNS Dynamic structure and clinical correlations [J].

Benarroch, Eduardo E. .

NEUROLOGY, 2015, 85 (16) :1417-1427

[8] MOLECULAR-DYNAMICS WITH COUPLING TO AN EXTERNAL BATH [J].

BERENDSEN, HJC ;

POSTMA, JPM ;

VANGUNSTEREN, WF ;

DINOLA, A ;

HAAK, JR .

JOURNAL OF CHEMICAL PHYSICS, 1984, 81 (08) :3684-3690

[9] Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gels [J].

Berns, Eric J. ;

Sur, Shantanu ;

Pan, Liuliu ;

Goldberger, Joshua E. ;

Suresh, Sunitha ;

Zhang, Shuming ;

Kessler, John A. ;

Stupp, Samuel I. .

BIOMATERIALS, 2014, 35 (01) :185-195

[10] Advances and Current Challenges Associated with the Use of Human Induced Pluripotent Stem Cells in Modeling Neurodegenerative Disease [J].

Berry, Bonnie J. ;

Smith, Alec S. T. ;

Young, Jessica E. ;

Mack, David L. .

CELLS TISSUES ORGANS, 2018, 205 (5-6) :331-349

← 1 2 3 4 5 6 7 8 9 10 →