Oxymatrine Inhibits Malignant Behaviors of Breast Cancer Cells by Inhibiting miR-188 Expression to Up-Regulate Phosphatase and Tensin Homolog (PTEN)

被引：0

作者：

Wang, Xiaobo ^{[1
]}

Hu, Yili ^{[1
]}

Chen, Diandian ^{[1
]}

Cheng, Le ^{[1
]}

Yu, Lili ^{[1
]}

Yang, Quanjun ^{[1
]}

机构：

[1] China Three Gorges Univ, Affiliated Renhe Hosp, Dept Oncol, Yichang 443000, Hubei, Peoples R China

来源：

JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2023年 / 13卷 / 01期

关键词：

BreastCancer; Oxymatrin; DeliveredmiR-188; byPTEN; IngentaProliferation; RESISTANCE;

D O I：

10.1166/jbt.2023.3220

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Oxymatrine has been applied to anti-cancer therapies for various cancers. The present study aimed to investigate the potential impact of miR-188 on breast cancer (BC) cell progression and underlying mechanism. After establishment of a rat model of BC, rats were administered with oxymatrine (4 mg/kg, 8 mg/kg), Xihuang pill (XH) (positive control), and miR-188 mimic (1 mg/kg) followed by analysis of tumor growth, the expression of miR-188, MMP-9, MMP-2, and PTEN, and BC cell behaviors. Oxymatrine significantly decreased tumor incidence and reduced tumor mass (p < 0.05) with 8 mg/kg intervention group and positive control group exhibiting higher tumor inhibition rate (p < 0.05). In addition, oxymatrine or XH effectively reduced cell proliferation, invasion and migration rate. Of note, compared to 4 mg/kg oxymatrine, 8 mg/kg oxymatrine and XH showed more significantly inhibitory effects on BC cells. Moreover, oxymatrine or XH significantly downregulated miR-188, MMP-9, and MMP-2 and upregulated PTEN. Mechanically, PTEN was indicated as the target of miR-188 with specific binding between them. In conclusion, Oxymatrine inhibits BC cell behaviors through down-regulation of miR-188 to increase PTEN expression. This study might provide a new basis for the management of BC. IP: 8.46.247.10 On: Thu, 09 Feb 2023 08:22:22

引用

页码：143 / 150

页数：8

共 27 条

[1] Acquired Resistance to KRASG12C Inhibition in Cancer [J].

Awad, M. M. ;

Liu, S. ;

Rybkin, I. I. ;

Arbour, K. C. ;

Dilly, J. ;

Zhu, V. W. ;

Johnson, M. L. ;

Heist, R. S. ;

Patil, T. ;

Riely, G. J. ;

Jacobson, J. O. ;

Yang, X. ;

Persky, N. S. ;

Root, D. E. ;

Lowder, K. E. ;

Feng, H. ;

Zhang, S. S. ;

Haigis, K. M. ;

Hung, Y. P. ;

Sholl, L. M. ;

Wolpin, B. M. ;

Wiese, J. ;

Christiansen, J. ;

Lee, J. ;

Schrock, A. B. ;

Lim, L. P. ;

Garg, K. ;

Li, M. ;

Engstrom, L. D. ;

Waters, L. ;

Lawson, J. D. ;

Olson, P. ;

Lito, P. ;

Ou, S. -H. I. ;

Christensen, J. G. ;

Janne, P. A. ;

Aguirre, A. J. .

NEW ENGLAND JOURNAL OF MEDICINE, 2021, 384 (25) :2382-2393

[2]

Ayyildiz A., 2021, Experimental Oncology, V43, P125, DOI [10.32471/exp-oncology.2312-8852.vol-43-no-2.16096, 10.32471/exp-oncology.2312-8852.vol-43-no-2.16096]

[3] PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys [J].