High-Intensity Exercise Training Alters the Effect of N-Acetylcysteine on Exercise-Related Muscle Ionic Shifts in Men

被引:4
作者
Lemminger, Anders K. K. [1 ]
Fiorenza, Matteo [1 ]
Eibye, Kasper [1 ]
Bangsbo, Jens [1 ]
Hostrup, Morten [1 ]
机构
[1] Univ Copenhagen, Dept Nutr Exercise & Sports, August Krogh Sect Human Physiol, DK-1165 Copenhagen, Denmark
关键词
ROS; oxygen species; scavengers; NAC; high-intensity training; potassium; lactate; pH; antioxidant; performance; CROSS-SECTIONAL AREA; SKELETAL-MUSCLE; EXHAUSTIVE EXERCISE; PROLONGED EXERCISE; ENERGY-PRODUCTION; FATIGUE; VOLUME; INFUSION; KINETICS; REST;
D O I
10.3390/antiox12010053
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study investigated whether high-intensity exercise training alters the effect of N-acetylcysteine (a precursor of antioxidant glutathione) on exercise-related muscle ionic shifts. We assigned 20 recreationally-active men to 6 weeks of high-intensity exercise training, comprising three weekly sessions of 4-10 x 20-s all-out bouts interspersed by 2 min recovery (SET, n = 10), or habitual lifestyle maintenance (n = 10). Before and after SET, we measured ionic shifts across the working muscle, using leg arteriovenous balance technique, during one-legged knee-extensor exercise to exhaustion with and without N-acetylcysteine infusion. Furthermore, we sampled vastus lateralis muscle biopsies for analyses of metabolites, mitochondrial respiratory function, and proteins regulating ion transport and antioxidant defense. SET lowered exercise-related H+, K+, lactate(-), and Na+ shifts and enhanced exercise performance by approximate to 45%. While N-acetylcysteine did not affect exercise-related ionic shifts before SET, it lowered H+, HCO3-, and Na+ shifts after SET. SET enhanced muscle mitochondrial respiratory capacity and augmented the abundance of Na+/K+-ATPase subunits (alpha(1) and beta(1)), ATP-sensitive K+ channel subunit (Kir6.2), and monocarboxylate transporter-1, as well as superoxide dismutase-2 and glutathione peroxidase-1. Collectively, these findings demonstrate that high-intensity exercise training not only induces multiple adaptations that enhance the ability to counter exercise-related ionic shifts but also potentiates the effect of N-acetylcysteine on ionic shifts during exercise.
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页数:24
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